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Study on Regenerating Shoots from Cotyledon Segment Cultures in Osmanthus fragrans(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年09期
Page:
126-128
Research Field:
Publishing date:

Info

Title:
Study on Regenerating Shoots from Cotyledon Segment Cultures in Osmanthus fragrans
Author(s):
WANG Zhen-hua1; PANG Ji-liang1; CHEN Yi-hong1; DING Xu-sheng2; SHEN Bai-chun2; CHEN Bo-xiang2
1.College of Life and Envirement Science，Hangzhou Normal University，Hangzhou，Zhejiang 310036;
2.Hangzhou Landscape Garden Engineering Corporation，Hangzhou，Zhejiang 310020
Keywords:
Osmanthus fragrans; cotyledon segments; in vitro culture; adventitious bud differentiation; thidiazuron
PACS:
S 685.13
DOI:
-
Abstract:
Cotyledon segments of Osmanthus fragrans ‘Dahuajingui’ were used as explant，the effect of hormone levels on the callus induced of cotyledons sliced of osmanthus,shoot differentiation and tube seedlings rooting were studied.The results showed that adventitious buds were differentiated from cotyledon segment calli after subculturing 9 times on differentiated medium （1/2MS+0.5 mg/L TDZ+0.1 mg/L NAA）Differentiation rate of the adventitious bud was 22.86%,and average number of.regenerating shoots was 3.22.After cotyledon segment calli were subcultured 10 times on same differentiated medium，frequency of the adventitious bud differentiation was up to 36%.The optimum rooting medium was 1/2MS+0.5 mg/L IBA+2.0 mg/L NAA,and the rooting rate could reach 70%.

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Memo

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Last Update: 2014-09-02