Infrared Spectrum Analysis of Extracts of Panax notoginseng Stems and Leaves(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Infrared Spectrum Analysis of Extracts of Panax notoginseng Stems and Leaves

Author(s):: ZHANG Zhi-xin; ZHANG Shi-xiu; Department of Biochemistry,Wenshan University，Wenshan Biological Resources Research and Development Centre，Wenshan，Yunnan 663000

Keywords:: Panax notoginseng stems and leaves; extracts; infrared spectroscopy

Abstract:: Taking Panax notoginseng stems and leaves as the materials，different elution fractions were prepared by precipitating with alcohol extracting-water，adsorption with D101 macroporous resin and gradient elution with ethanol solution.Then analyzing ‘macro fingerprint characteristics’ of different elution fractions by Fourier Transform infrared spectroscopy and second serivative infrared spectroscopy.The results were compared with infrared spectroscopy ofprotopanaxadiol saponins Rb1 reference substance，flavonoids quercetin reference substance and rutin.The results showed that one-dimensiona infrared spectroscopy and second serivative infrared spectroscopy of different elution fractions were different.The characteristic absorption peaks of different elution fractions were described.The elution fractions with 30% and 50% ethanol solution mainly were different component saponins of Panax notoginseng stems and leaves.The elution fractions with 60%，70% and 90% ethanol solution mainly were flavonoids with different structure.These products can be used as different pharmacological component to meet the challenge of drug discovery and establishing analysis pattern for extraction of Panax notoginseng stems and leaves crude drug.

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Last Update: 2014-08-31