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Bioinformatics Analysis of Betaine Aldehyde Dehydrogenase in Plants(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年08期
Page:
120-124
Research Field:
Publishing date:

Info

Title:
Bioinformatics Analysis of Betaine Aldehyde Dehydrogenase in Plants
Author(s):
NONG Qing-yuan1MAI Xiu-ying1ZHOU Hong1FENG Qian1CHEN Jin-song2LIAO Hai1 ZHOU Jia-yu1
1.College of Life Science and Engineering,Southwest Jiaotong University,Chengdu,Sichuan 610031;
2.Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu,Sichuan 610041
Keywords:
betaine aldehyde dehydrogenasebioinformaticsGenBankresistance to stress
PACS:
Q 71;Q 949.93
DOI:
-
Abstract:
Bioinformatic methods were employed to analyze the amino acid sequences of Betaine Aldehyde Dehydrogenase (BADH) from Genbank database,such as Beta vulgaris, Spinacia oleracea, Atriplex hortensis, Suaeda liaotungensis, Zea mays,etc.The results showed that BADHs were stable proteins.Ala and Glu were abundant in those BADHs.Sequence alignment revealed that BADHs were highly homologous in different plants,and the residues involved with function were very conservative.BADHs had been found to be targeted to different subcellular compartments.BADHs could be used as genetic differentiation and an important basis for the study of molecular evolution.There was no signal peptide in segmental plants.BADHs had no transmembrane structure and could be phosphorylated by protein kinase.α-helix were the major structural element of polypeptide chain.Meanwhile,BADHs contained typical function domains of ALDH.This study provided a theoretical basis for both the enzymatic mechanism of BADHs and the plant resistance to environmental stress.

References:

[1]Fitzgerald T L,Waters D L E,Henry R J.Betaine aldehyde dehydrogenase in plants [J].Plant Biol,2009,11:119-130.

[2]Yang X,Liang Z,Wen X,et al.Genetic engineering of the biosynthesis of glycine betaine leads to increased tolerance of photosynthesis to salt stress in transgenic tobacco plants [J].Plant Mol.Biol.,2008,66:73-86.
[3]Park E J,Jeknic Z,Sakamoto A,et al.Genetic engineering of glycine betaine synthesis in tomato protects seeds,plants,and flowers from chilling damage [J].Plant J.,2004,40:474-487
[4]Weretilnyk E A,Hanson A D.Molecular cloning of a plant betainealdehyde dehydrogenase,an enzyme implicated in adaptation to salinity and drought [J].Proc.Natl.Acad.Sci.,1990,87:2745-2749.
[5]Goel D,Singh A K,Yadav V,et al.Overexpression of osmotin gene confers tolerance to salt and drought stresses in transgenic tomato (Solanum lycopersicum L.) [J].Protoplasma,2010,245(1-4):133-141.
[6]Oishi H,Ebin M.Isolation of cDNA and enzymatic properties of betaine aldehyde dehydrogenase from Zoysia tenuifolia [J].Journal of Plant Physiol,2005,162:1077-1086.
[7]Gardiner J,Schroeder S,Polacco M L,et al.Anchoring 9 371 maize expressed sequence tagged unigenes to the bacterial artificial chromosome contig map by two-dimensional overgo hybridization [J].Plant Physiol.,2004,134(4):1317-1326.[8]Kumar S,Dhingra A,Daniell H.Plastid-expressed betaine aldehyde dehydrogenase gene in carrot cultured cells,roots,and leaves confers enhanced salt tolerance [J].Plant Physiol.,2004,136:2843-2854.
[9]Trossat C,Rathinasabapathi B,Hanson A D.Transgenically expressed betaine aldehyde dehydrogenase efficiently catalyzes oxidation of dimethylsulfoniopropionaldehyde and [omega]-aminoaldehydes[J].Plant Physiol,1997,113:1457-1461.
[10]Chen T H H,Murata N.Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes[J].Curr.Opin.Plant Biol.,2002,5:250-257.
[11]Zhang F L,Niu B,Wang Y C,et al.A novel betaine aldehyde dehydrogenase gene from Jatropha curcas,encoding an enzyme implicated in adaptation to environmental stress [J].Plant Sci.,2008,174(5):510-518.
[12]Nakamura T,Nomura M,Mori H,et al.An isozyme of betaine aldehyde dehydrogenase in barley [J].Plant Cell Physiol,2001,42(10):1088-1092.(该文作者还有周嘉裕,工作单位同第一作者。)

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Last Update: 2014-08-31