In vitro Rapid Propagation of Weigela florida cv.Red Prince Stem-segment(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: In vitro Rapid Propagation of Weigela florida cv.Red Prince Stem-segment

Author(s):: LI Fang; REN Xue-qin; SUN Yang-wu; ZHU Yuan-di; ZHANG Wen; College of Agriculture and Biotechnology，China Agricultural University,Key Laboratory of Beijing Municipality’s Stress Physiology and Molecular Biology for Fruit Trees，Beijng 100193

Keywords:: Weigela florida cv.Red Prince; propagation; plant growth regulator

Abstract:: The stem-segments with axillary buds of Weigela florida cv. Red Prince were used as the explant.MS was used as basic medium to carry out in vitro culture to select optimal medium，also the best sterilization method and time were selected.The results indicated that 0.1% HgCl2 for 7 min was the best way.Its effects were better than 10% NaClO.The appropriate cultured medium for regeneration was MS+BA 2.0 mg/L+NAA 0.1 mg/L+KT 2.0 mg/L and the best root-induced cultured medium was 1/2MS+IBA 0.6 mg/L.All of the complete plants with roots were grown in the soil were survived.

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Last Update: 2014-08-31