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Study on Tissue Culture and Rapid Propagation of Curcuma attenuta(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年07期
Page:
142-144
Research Field:
Publishing date:

Info

Title:
Study on Tissue Culture and Rapid Propagation of Curcuma attenuta
Author(s):
KOU Ya-ping; ZHANG Shi-jun; XIONG You-hua; LIU Nian
College of Horticultural and Landscape Architecture，Zhongkai University of Agriculture and Engineering，Guangzhou，Guangdong 510225
Keywords:
Curcuma attenuta; axillary bud; tissue culture and rapid propagation
PACS:
S 682.2+9
DOI:
-
Abstract:
Taking rhizomes axillary buds of Curcuma attenuata as explants with adding 6-BA,NAA,TDZ different concentration and various combinations of MS basic medium,the optimal conditions of tissus culture and rapid propagation technology about shooting induction,multiple shoot subculture,proliferation,rooting,and seddling cultivation were studied.The results showed that the optimal medium for shooting induction was MS+6-BA 2.0 mg/L+NAA 0.5 mg/L with an inducing rate of 86.7% and rapid growth.The proliferation efficiency of adventitious shoots increased 14.1-fold after subculture for three times on MS medium containing 2.0 mg/L 6-BA and 0.1 mg/L TDZ.Medium containing 1.0 mg/L 6-BA and 0.5 mg/L NAA served as the rooting medium.Over 90% of the plantlets survived when they grow more than 6 cm tall.

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Last Update: 2014-09-03