|Table of Contents|

Detection of Strawberry Mottle Virus with RT-PCR(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年07期
Page:
132-134
Research Field:
Publishing date:

Info

Title:
Detection of Strawberry Mottle Virus with RT-PCR
Author(s):
QUE Zhi-qun
College of Life Sciences and Resources Environment Sciences,Yichun University,Yichun，Jiangxi 336000
Keywords:
strawberry mottle virus; primer; CTAB; RNA; RT-PCR; specific fragment
PACS:
S 436.639
DOI:
-
Abstract:
Special primers were design based on the sequence of coat protein which was a conservative region of strawberry mottle virus.Highly pure total RNA including strawberry and strawberry mottle virus was extracted with modified CTAB method.Reverse transcription-polymerase chain reaction (RT-PCR ) was carried out in order to establish detection system of strawberry mottle virus.The results showed that a good RNA quality was obtained and modified CTAB method was feasible for extraction of total RNA of strawberry.

References:

[1]王国平,刘福昌.带病毒和无病毒草莓生长和结果的比较[J].北方果树,1990(3):9-12.

[2]韦石泉,吴元华.我国草莓斑驳病毒研究鉴定[J].植物病理学报,1994,24(4):293-298．
[3]周厚成,何水涛.草莓病毒病研究进展[J].果树学报,2003,20(5):423-426.
[4]贾慧,王进忠,陈忠斌,等.百合病毒检测技术研究进展[J].北京农学院学报,2004,19(4）：73-76.
[5]王正华,邵永春,尹涛.草莓病毒的检测方法及症状识别[J].山东农业科学,1999(1):31-33.
[6]杨洪一,张志宏,杜国栋,等.利用内标为基础的RT-PCR技术检测草莓斑驳病毒[J].植物病理学报,2005,35(2):116-122.
[7]Thompson J R,Leone G,Lindner J L,et a1.Characterization and complete nucleotide sequence of Strawberry mottle virus：a tentative member of a new family of bipartite plant picorna-like viruses [J].Journal of General Virology,2002,83(1):229-239.
[8]Chang S,Puryear J,Cairney J.A simple and efficient method for isolating RNA from pine trees [J].Plant Molecular Biology Reporter,1993,11(2):114-117．
[9]Bekesiova I,Nap J P,Mlynaroval L.Isolation of high quality DNA and RNA from leaves of the Carnivorous plant Drosera rotundifolia[J].Plant Molecular Biology Reporter,1999,17(3)：269-277．
[10]程水源,陈昆松,杜何为,等.银杏RNA的提取[J].果树学报,2005,22(4):428-429.

Memo

Memo:
-
Last Update: 2014-09-03