|Table of Contents|

Study on Isolation and Culture Characteristics of Paphiopedilum micranthum Mycorrhizal Fungi(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年07期
Page:
61-64
Research Field:
Publishing date:

Info

Title:
Study on Isolation and Culture Characteristics of Paphiopedilum micranthum Mycorrhizal Fungi
Author(s):
TIAN Fan1; ZHU Guo-sheng 2; 3; GUI Yang 2; 3; BAI Xin-xiang1
1.Guizhou University，Guiyang，Guizhou 550025;
2.Institute of Morden Chinese Medical Materials，Guizhou Academy of Agricaltural Sciences，Guiyang，Guizhou 550006；
3.Guizhou Key Laboratory of Agricultural Biotechnology，Guiyang，Guizhou 550006
Keywords:
Paphiopedilum micranthum; mycorrhizal fungi; peloton; isolation; culture
PACS:
S 682.31
DOI:
-
Abstract:
Eight mycorrhizal endophyte strains were isolated from a single peloton of Paphiopedilum micranthum.After purification，the strains were cultured by the hole method and the morphological characteristics were observed.At the same time，the growth rate was determinated.Isolated strains were named as DJYYDL001,DJYYDL003,DJYYDL004，DJYYDL006，DJYYDL008,DJYYDL009,DJYYDL011,DJYYDL015.The results showed that the single sclerotium isolated from the pelotons were cultured in thermostatic,after 12 hours，microscopy and absorb the hyphae grow group was the best time.Without mercuric chloride treatment root sclerotium pollution rate，while processing 2~3 min posterior root segment sclerotium contamination rate minimum.

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Memo

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Last Update: 2014-09-03