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Research on the Embryo Rescue of Stenospemocarpic Seedless Grape(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年07期
Page:
1-5
Research Field:
Publishing date:

Info

Title:
Research on the Embryo Rescue of Stenospemocarpic Seedless Grape
Author(s):
NIU Ru-xuan; ZHANG Jian-xia; WANG Yue-jin; ZHAO Kai
College of Horticulture，Northwest Agriculture and Forestry University，State Key Laboratory of Crop Stress Biology in Arid Area，Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China，Shaanxi Province Agriculture Molecular Biology Laboratory，Ministry of Agriculture of China，Yangling，Shaanxi 712100
Keywords:
stenospemocarpic; seedless grapes; embryo rescue; sampling time; medium; screening
PACS:
S 663.103.6
DOI:
-
Abstract:
To improve the embryo development and germination in embryo rescue,taking stenospemocarpic seedless grapes of ‘Sultanina Rose’，‘Perlette’,‘Autumn Royal’ as test materials，the best timing for embryo rescue of the three species under natural pollination conditions were investigated,and the suitable media were selected for the cultivation of embryo culture.The results showed that the best sampling time for ‘Sultanina Rose’，‘Perlette’，‘Autumn Royal’were 51～53 d，55 d and 66 d after full bloom respectively.The optimum media that selected was MM4+0.5 mg/L GA3+1.5 mg/L IAA+500 mg/L hydrolyzed casein+60.0 g/L sucrose+1.5 g/L activated carbon+10.0 μmol/L ZnSO4+1.0 mmol/L glycine+1.0 mmol/L cysteine+100.0 mmol/L mannitol.The development rate and germination rate of ‘Sultanina Rose’，‘Perlette’and ‘Autumn Royal’s’ovules was 15.00% and 6.67%，24.00% and 10.00%，10.00% and 5.00% respectively，and an average of 16.33% and 7.22%.

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Last Update: 2014-09-03