Studies on Induced Calli and Plant Regeneration of Caralluma hesperidum(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Studies on Induced Calli and Plant Regeneration of Caralluma hesperidum

Author(s):: DONG She-qin; YANG Ya-zhen; YANG Deliang; YE Kai-wen; TIAN Zhi-hong; College of Life Science,Yangtze University,Jingzhou,Hubei 434025

Abstract:: The axillary bud of a stem tuber,non-axillary bud of astem tuber and spiked,were cultured in order to study the factors affecting their regeneration and calli,such as density and combination of plant growth regulator,culture medium,days of dark culture and explant.The results showed that the best explant to induced calli was non-axillary bud of astem tuber，and the calli rato was 90% when they were cultured with No.1.Maijin+CH+6-BA 1.0～1.8 mg/L+2,4-D0.5～2.0 mg/L and 6 d of days in the dark，besides，compact quality and a certain extent looes，were built up best material of cell asexual line.The best explant to induced clumped bud was axillary bud of a stem tuber,and the bud rato was 91% when the axillary bud of astem tuber were cultured with H+TDZ 4 mg/L+NAA 0.4 mg/L.The best culture medium of adventitious bud strong was improved H+TDZ 4 mg/L+NAA 0.4 mg/L.The best culture medium rooting was 1/2MS+NAA 0.5 mg/L.

[1]黄清俊,丁雨龙，谢维荪,等.多肉植物离体种质研究的迫切性、可行性及研究现状[J].上海农业学报,2003,19(1）：41-45.

[2]高山林,朱丹妮,徐德然,等.组织培养技术的研究[J].药用生物技术,1995,2(4):33-36.

[3]李俊明.植物组织培养教程[M].北京：中国农业大学出版社,2002:25-28.

[4]程家胜.植物组织培养与工厂化育苗技术[M].北京:金盾出版社,2003:105-113.

[5]潘瑞炽.植物生理学[M].北京：高等教育出版社,2001:169-205.[6]廖祥儒.施NO₃-和NH₄⁺与植物抗盐性关系[J].土壤肥料,1997(1):14-16.

[7]姜明兰,刘声远.组织培养生产高山红景天有效成分的研究[J].沈阳农业大学学报,1994,25（4）：355-359.[8]郑光植.药用植物组织培养在工业生产上应用研究的进展[J].植物生理学通讯,1980(4):1-12.

[9]周维燕.植物细胞工程原理与技术[M].北京:中国农业大学出版社,2001:60-66.

Last Update: 2014-08-30