Study on the Tissue Culture System of Tagetes erecta L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: ZHENG Jie; LI Su-qin; HUANG Hong-mei; College of Oriental Science and Technology，Hunan Agricultural University,Changsha，Hunan 410128

Abstract:: Using leaves and stems as explants of Tagetes erecta L.，the optimal medium at different stages of the tissue culture was studied to establish the tissue culture system of Tagetes erecta L..The results showed that the leaves were fit for inducing callus.In MS medium with 6-BA 2.0 mg/L and NAA 0.2 mg/L，the callus induction rate was the highest.The optimum medium of the adventitious bud regeneration was MS medium with 6-BA 1.0 mg/L and NAA ?0.1 mg/L.In 1/2MS medium with IBA 1.0 mg/L and IAA 0.8 mg/L,the root could be induced easily.

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Last Update: 2014-08-30