Study of Tissue Culture Technique of Salvia miltiorrhiza f.alba(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: QI Cui-ying¹; LI Xu-xia¹; WANG Ning²; WANG Feng-xiang¹; ZHANG Fu-xin¹; 1.Institute of Biotechnology,Laiwu Vocational and Technical College,Laiwu,Shandong 271100;
2.Laiwu Forestry Bureau,Laiwu,Shandong 271100

Keywords:: Salvia miltiorrhiza f.alba; callus; vitrification preventing; root

Abstract:: Leaves of Salvia miltiorrhiza.f.alba were used as explant,the effects of the explants variations,ratio of steroids,concentration of agar and active carbon on tissue culture and propagation were studied.The results indicated that the media of MS+6-BA 2.0 mg/L+NAA 1.0 mg/L+agar 7% fit the inducing of two types of calli,the MS+KT 1.5 mg/L+NAA 0.5 mg/L+agar 8%+active carbon medium was most suitable for decreasing vitrification of plantlets and fit to grow up strong seedlings,the 1/2MS+IBA 0.2 mg/L medium(or 0.3% active carbon simultaneously) was better for root generation with rooting rate of 100%.The survival rate of transplantation of test-tube seedling could reach a high level at 95%.

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Last Update: 2014-08-30