|Table of Contents|

Establishment of Calli-mediated Regeneration System in Curcuma longa(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年06期
Page:
96-98
Research Field:
Publishing date:

Info

Title:
Establishment of Calli-mediated Regeneration System in Curcuma longa
Author(s):
ZHANG Shi-jun1; LIU Nian1; SHENG Ai-wu1; WU Guo-jiang2
1.College of Horticulture and Landscape Architecture,Zhongkai University of Agriculture and Engineering,Guangzhou，Guangdong 510225;
2.South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,Guangdong 510650
Keywords:
Curcuma longa; tissue culture; bud base; calli; regeneration
PACS:
S 567.23+9
DOI:
-
Abstract:
A protocol was investigated for callus induction using bud base from tissue-cultured Curcuma longa explants.The results showed that MS media supplemented with 2,4-D or with 2,4-D+6-BA could successfully induce calli formation at cut positions of explants.However，the calli grew slowly.The optimal calli induction medium was MS+0.5 mg/L TDZ+0.5 mg/L BA+0.3 mg/L 2,4-D，which showed the highest induction rate and calli growing mass，with an inducing rate of 80% and growing mass of 148.6 mg per month.The optimal plantlet regeneration medium was MS+0.5 mg/L TDZ+2.0 mg/L BA+0.2 mg/L NAA，with a regeneration rate of 90% and the average shoot number of 5.1 per explant.Regenerated plantlets (5 cm at length) were transferred to soil in greenhouse.More than 90% of plantlets could survive.

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Last Update: 2014-08-30