|Table of Contents|

Optimization of an ISSR-PCR Reaction System of Chinese Cabbage by Orthogonal Design(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年05期
Page:
127-129
Research Field:
Publishing date:

Info

Title:
Optimization of an ISSR-PCR Reaction System of Chinese Cabbage by Orthogonal Design
Author(s):
WU Chun-yan; GAO Yi; SONG Ting-yu; ZHANG Xiao-ming; HAN Yu-zhu
College of Horticulture,Jilin Agricultural University,Changchun,Jilin 130118
Keywords:
Chinese cabbage; ISSR; orthogonal design; PCR reaction system
PACS:
S 634.103.6
DOI:
-
Abstract:
Chinese cabbage genomic DNA,extracted from its fresh young leaves by DNA extraction kit was applied to optimize ISSR-PCR amplification system based on orthogonal design.Five factors were considered such as dNTP，Mg2+，Taq- DNA polymerase，primer and template DNA at four levels.A suitable ISSR-PCR reaction system was established.The results showed that 25 μL reaction system containing 1.5 mmol/L Mg2+，200 μmol/L dNTP，0.5 U Taq-DNA polymerase,0.7 μmol/L primer and 30 ng DNA template.The number of cycles was also investigated.Three different cycle numbers of were used to test the optimized reaction system.The results indicated that the appropriate cycle numbers of the optimized reaction was 30.

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Last Update: 2014-08-27