|Table of Contents|

A Primary Study on Tissue Culture of Camelia sinensis cv.Chongqingpipacha(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年05期
Page:
122-124
Research Field:
Publishing date:

Info

Title:
A Primary Study on Tissue Culture of Camelia sinensis cv.Chongqingpipacha
Author(s):
HU Hong-sha; TANG Lin
College of Life Science,Sichuan University，Chengdu，Sichuan 610064
Keywords:
Camelia sinensis cv.Chongqingpipacha; sterilization; callus induction; proliferation
PACS:
S 571.1
DOI:
-
Abstract:
Using Camelia sinensis cv.Chongqingpipacha buds as materials，explants sterilization，callus induction，and proliferation culture of buds were studied.The results showed that using cutting seedling in culturing room as explants，the optimal sterilization time was about 3 minutes with 0.05% HgCl2after the 10 minutes with 0.1% HgCl2，and its survival rate was about 88.5%.The optimal induction of callus was MS+1 mg/L BA+0.5 mg/L NAA.The callus color was bright green，and its texture was harder，then its surface turn to granular when was transferred to MS+（1,2 mg/L）TDZ.The best medium for proliferation of buds was MS+2 mg/L BA+0.05 mg/L IAA+0.5 mg/L GA3and MS+1 mg/L TDZ+1 mg/L GA3.The alternate cultivation on the two medium was in favor of proliferation of buds.

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Last Update: 2014-08-27