|Table of Contents|

Preliminary Study on the Tissue Culture of Terminal Bud of Peony(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年04期
Page:
131-133
Research Field:
Publishing date:

Info

Title:
Preliminary Study on the Tissue Culture of Terminal Bud of Peony
Author(s):
YOU Yang1JIA Wen-qing1WANG Hui2
1.College of Horticulture and Landscape Architecture,Henan Institute of Science and Technology,Xinxiang,Henna 453003;
2.Center of Rural Economic Development Service of Wujiadian Countryside Shihe Area,Xinyang,Henan 464142
Keywords:
peonyterminal budculture
PACS:
S 685.11
DOI:
-
Abstract:
Using the terminal bud of peony as explants,the effect of 0.1% HgCl2 with different treatment time to pollution rate,browning rate and survival rate of terminal bud,and with MS as the basic medium,then supplemented with different plant growth regulator to germinating of terminal bud,induction on multiple shoot and rooting of peony were studied.The results showed that with 0.1% HgCl2 treating the terminal bud of penoy,the optimal treating about eight minutes.The medium of optimal of germinating of terminal bud and induction on multiple shoot was MS+Ca2++6-BA 2.5 mg/L+NAA 0.5 mg/L.In this test,on the 50th days,the rooting medium induced the rooted ratio was 18%,the effect was not well,shoud be further studied.

References:

[1]刘淑敏,王莲英,吴涤新.等.牡丹[M].北京:中国建筑工业出版社,1987.
[2]杨红超,裴冬利.牡丹种子胚培养研究[J].广西农业科学,2006,37(2):108-110.
[3]孔祥生,张妙霞.牡丹离体快繁技术研究[J].北方园艺,1998,3(4):87-89.
[4]周仁超,姚崇怀.紫斑牡丹胚培养与植株再生[J].亚热带植物科学,2001,30(3):60.
[5]张桂花,王洪梅,王连祥.牡丹组织培养技术研究[J].山东农业科学,2001(5):16-18.
[6]何桂梅,成仿云,李萍.两种牡丹胚珠与幼胚离体培养的初步研究[J].园艺学报,2006,33(1):185.
[7]范小峰,郭小强,马世荣.三种牡丹组织培养比较研究[J].北方园艺2010(4):132-134.
[8]何松林,陈莉,陈笑蕾.牡丹鳞芽诱导与增殖过程中影响因子研究[J].河南农业大学学报,2009,10(5):511-516.
[9]张启翔.中国观赏园艺研究进展2009[M].北京:中国林业出版社,2009:258-260.
[10]李志军,刘志国,李红梅.牡丹组培快繁技术研究[J].山东林业科技,2006(3):39-40.
[11]谭文澄,戴策刚.观赏植物组织培养技术[M].北京:中国林业出版社,1997:278.
[12]王利民,周毅,陈龙友,等.植物组织培养中消毒剂的运用.贵州师范大学学报(自然科学版),2002,2(1):15-17.
[13]贾文庆,刘会超.‘凤丹’胚不定芽诱导和生根研究[J].北方园艺,2009(3):69-71.

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Last Update: 2014-08-26