|Table of Contents|

Effects of Different Concentrations of TDZ on Embroyogenic Callus Inductionand Differentiation of Hypericum perforatum L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年03期
Page:
163-165
Research Field:
Publishing date:

Info

Title:
Effects of Different Concentrations of TDZ on Embroyogenic Callus Inductionand Differentiation of Hypericum perforatum L.
Author(s):
WANG Fu-yuan1; TANG Ning2; SONG Dong-jie2
1.School of Higher Vocational and Technical，China Pharmaceutical University，Nanjing，Jiangsu 211198；
2.School of Biochemistry and Environmental Engineering，Nanjing Xiaozhuang College，Nanjing，Jiangsu 211171
Keywords:
Hypericum perforatum L.; TDZ; callus; adventitious bud
PACS:
S 567.23+9
DOI:
-
Abstract:
The embryogenic callus induction and shoot regeneration of Hypericum perforatum L.were studied systematically through different concentrations of TDZ.The results indicated that the most suitable part for inducing embryogenic callus was the petiole.The inducing rate reached as high as 100%.The optimized medium for inducing embryogenic callus and plant regeneration was MS+0.05 mg/L TDZ+0.2 mg/L NAA.The adventitious buds differentiating rate was 55%.The size of callus can increase greatly in the first subculture on the optimized inducing medium and the average number of green-coloured masses of embryogenic cells on each callus was 5.6.On the second and third subculture，callus stopped growing further and the number of masses of embryogenic cells decreased gradually.

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Last Update: 2014-08-27