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Effect of Bacterial Concentration on PCR Amplification(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年03期
Page:
127-129
Research Field:
Publishing date:

Info

Title:
Effect of Bacterial Concentration on PCR Amplification
Author(s):
HAO Ai-ping; WEI Ji-cheng; GUO Hui-yan
College of Life Sciences and Technology，Mudanjiang Normal College，Mudanjiang,Heilongjiang 157012
Keywords:
bacterial concentration; template; Polymerase chain reaction
PACS:
Q 949.3
DOI:
-
Abstract:
Taking Escherichia coli and Agrobacterium were studied，in which included Chitinase gene as materials the effect of bacterial concentration on Polymerase chain reaction.The results showed that the band would appear when the bacterial OD value increased to 0.006 and then took it as the template for PCR by electrophoresis，meanwhile，the electrophoresis bands became more and more brighter with increasing of the OD value；When the OD value was between 0.200 to 0.700；The electrophoresis bands were quiet clear；The ingredients in the liquid of LB medium failed to effect on DNA template，and the bacterial DNA could be amplified as normal as usual.

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Last Update: 2014-08-26