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Establishment of Tissue Culture Regeneration System of Populus tomentosa Clone-30(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年03期
Page:
110-113
Research Field:
Publishing date:

Info

Title:
Establishment of Tissue Culture Regeneration System of Populus tomentosa Clone-30
Author(s):
LI HuiFAN Jun-fengGAO Jian-sheZHOU Yong-xueWANG Hu
College of Forestry,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100
Keywords:
populous tomentosa colne-30proliferationrootagetissue culture
PACS:
S 792.117
DOI:
-
Abstract:
Taking stems of the P.tomentosa clone-30 as explants, the regeneration ability and conditions were studied,in order to build a rapid tissue propagation system.The results showed that the appropriate basic medium was MS and the appropriate inductive medium was MS+6-BA 0.5 mg/L and MS+TDZ 0.0015 mg/L+NAA 0.01 mg/L,the shoot induction rates were respectively 86.7% and 83.3%.The optimal regeneration medium was MS+6-BA 0.7 mg/L+NAA 0.05 mg/L and the regeneration rate was 628%,the optimal rootage medium was 1/2MS+NAA 0.01 mg/L,and the rate of the rooting was 96.8%。

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Last Update: 2014-08-26