|Table of Contents|

A MicroRNA-Based Gene Silencing Vector Construction Method in Tomato(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年03期
Page:
101-104
Research Field:
Publishing date:

Info

Title:
A MicroRNA-Based Gene Silencing Vector Construction Method in Tomato
Author(s):
WANG Yun-xiang; ZUO Jin-hua; JU Zheng; GAO Chao; ZHU Yi; ZHU Ben-zhong
College of Food Science and Nutritional Engineering，China Agricultural University，Beijing 100083
Keywords:
artificial microRNA; gene silencing; tomato; vector construction
PACS:
S 641.203.6
DOI:
-
Abstract:
A microRNA-baesd method for easy and rapid construction of tomato miRNA vector was described.The plasmid PRS300 which contained the miR319a precursor was used as the temple，the overlapping PCR amplification for the miR1917 whose target gene was LeCTR1 which were key negative regulators of ethylene responses was processed，and the reconstructed precursor was successfully transformed to the expression vector pGreen0029-35s.The transgenic plants were successfully obtained by the leaf disc method mediated by Agrobacterium tumefaciens C58.This method could provide a theoretical basis for the miRNA mechanisms of action research in tomato.

References:

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Last Update: 2014-08-26