Study on Extraction and Purification Process of Polysaccharides in Neosinocalamus affinis (Rendle) Keng f. Leaves(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on Extraction and Purification Process of Polysaccharides in Neosinocalamus affinis (Rendle) Keng f. Leaves

Author(s):: GUO Lei; KAN Huan; FAN Fang-yu; LIU Yun; JIANG Jin-yan; SHEN Xue-jing; Southwest Forestry University,Kunming,Yunnan 650224

Keywords:: Neosinocalamus affinis (Rendle) Keng f.; polysaccharides; response surface methodology; decoloration; purification

Abstract:: Polysaccharides in Neosinocalamus affinis (Rendle) Keng f. levels was extracted by water to produce.Single-factor method was adopted to investigate the effects of extract-time,temperature and solid-liquid ratio on degree of extraction content of polysaccharides.Subsequently,a central composite design(CCD) involving 17 experiments of three variables(i.e.,extract-time,temperature and solid-liquid ratio) at three levels combined with response surface methodology was employed to attain the highest extraction content.The results showed that the optimal extract-time,temperature,solid-liquid ratio were 143 h,768℃,1∶803,respectively.On this condition,the content of polysaccharides was 411 mg/g.By the method of a series of purification process,the purity of polysaccharides was 6132%.

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Last Update: 2014-08-24