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Investigation and Preliminary Study of Elimination of CymMV and ORSV from Phalaenopsis in China(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年02期
Page:
137-140
Research Field:
Publishing date:

Info

Title:
Investigation and Preliminary Study of Elimination of CymMV and ORSV from Phalaenopsis in China
Author(s):
DING Lan; GUO Yan; DONG Gang; YANG Ling; ZHANG Li; LIU Guo-an
College of Life Science,Northwest Normal University,Lanzhou,Gansu 730070
Keywords:
DAS-ELISA; Phalaenopsis; Cymbidium mosaic virus; Odontoglossum ringspot virus; virus-free
PACS:
S 68231
DOI:
-
Abstract:
The study had detected two main virus,Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV) of seventeen strains（twenty two samples） of Phalaenopsis collected from different farms,with the method of double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA);Meanwhile,two ways were employed for Elimination of the viruses from four Phalaenopsis.The results showed that twenty samples were positive for two viruses (CymMV and ORSV),and only one sample was negative for two viruses (CymMV and ORSV).The virus-free rate of strain R-2-2 and strain Y-4-2 were 227% (CymMV) and 191% (ORSV),303% (CymMV) and 80% (ORSV) after shoot-tip culture,respectively;the virus-free rate of strain R-1-2 and strain R-11-1 were 254% (CymMV) and 14% (ORSV)，252% (CymMV) and 242% (ORSV) by induction protocorm,respectively.It indicated that the viruses from Phalaenopsis could not be eliminated thoroughly by shoot-tip culture or induction protocorm for only one time.

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Last Update: 2014-08-24