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Optimization of Chromosome Observing Method of Root-tip Tissue in Two Species of Araceae(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年02期
Page:
134-136
Research Field:
Publishing date:

Info

Title:
Optimization of Chromosome Observing Method of Root-tip Tissue in Two Species of Araceae
Author(s):
LI Qing-ling1; CHEN Rong1; 2; YANG Yue-sheng1; ZHU Chang-san2
1.Center for Medicinal Plant Research,College of Life Science,South China Agricultural University,Guangzhou,Guangdong 510642;
2.GuangxiEco-engineering Vocational and Technical College,Liuzhou,Guangxi 545004
Keywords:
Spathiphyllum floribundum; Alocasia indica Schott; chromosome observation
PACS:
S 68236
DOI:
-
Abstract:
Araceae plants Spathiphyllum floribundum and Alocasia indica Schott were used in this study to optimize the chromosome observation method of root-tip tissue.The results showed that both materials were better collected at the time period of 12:00~14:00.Spathiphyllum floribundum achieved better result with a pretreatment of 6 h in fridge at the temperature of 8℃ and Alocasia indica Schott reached optimum condition with a pretreatment of 8 h in fridge at the temperature of 16℃ when pretreated in saturated dichlorobenzene.Spathiphyllum floribundum and Alocasia indica Schott reached ideal results when respectively treated for 10 min and 15 min with HCl 1 mol/L for 40 min in water bath.White meristematic tissue at the root-tip was cut and put on glass slide for chromosome number observation and photographing after 20 min dyeing with carbolfuchsin.

References:

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Memo

Memo:
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Last Update: 2014-08-24