|Table of Contents|

Establishment of High Frequency Shoot Regeneration System of Cucumis melo(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年02期
Page:
132-133
Research Field:
Publishing date:

Info

Title:
Establishment of High Frequency Shoot Regeneration System of Cucumis melo
Author(s):
KONG Wei-ping; CHENG Hong
Institute of Vegetable,Gansu Academy of Agricultural Sciences,Lanzhou,Gansu 730070
Keywords:
Cucumis melo; explants; regeneration system
PACS:
S 6526036
DOI:
-
Abstract:
Cotyledon,hypocotyledonary axis,root tip,euphylla and adventitious buds inducement with various concentration of 6-BA of ‘G24’ and ‘Gantian No1’of Cucumis melos at the growing stage of 5~7 d were studied in this research,in order to establish stable tissue culture techniques for genetic transformation and to provide technical support.At the meantime,conditions for root growth and transplant were also primarily studied.The results showed that no significant difference on adventitious buds inducement with the two tested material.The adventitious buds inducement rate of ‘G24’was 972%,while ‘Gantian No1’ was 916%;cotyledon at the hypocotyl side was the optimum explants for tissue culture with over 95% of adventitious buds inducement rate and the optimum concentration of 6-BA was 10 mg/L.

References:

[1]陶兴林,黄永红,赵长增,等.厚皮甜瓜离体培养再生植株能力的基因型差异研究[J].果树学报,2005,22(3):252-255.

[2]孔维萍,程鸿,苏永全,等.西甜瓜组织培养与遗传转化影响因子研究进展[J].长江蔬菜,2010（12）:1-4.[3]Leshetn B.Polarity and responsive regions for regeneration in the cured melon cotyledon[J].Plant Physiol,1989,135:237-239.
[4]冯凤娟,梁栋,马锋旺等.甜瓜叶片高效再生体系的建立[J].西北农业学报,2008,17(5)：321-324.
[5]于喜艳,何启伟,孔庆国.甜瓜子叶组织培养的研究[J].山东农业科学,2002(2):22-23.
[6]王建设,陈杭.甜瓜再生芽高效诱导方法的研究[J].园艺学报,1999,26(5):339-340.

Memo

Memo:
-
Last Update: 2014-08-24