Study on the Tissue Culture Technology of the Seedling Propagation in Euphorbia pulcherrima Wild(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2012年02期

Page:

127-129

Research Field:

Publishing date:

Info

Title:

Study on the Tissue Culture Technology of the Seedling Propagation in Euphorbia pulcherrima Wild

Author(s):

SHAO Yuan-jian¹; 2; ZHOU Jiang-chen¹

1.Nantong Agricultural Vocational Technology College,Nantong,Jiangsu 226007;

2.Nantong Key Laboratory of Agricultural Biotechnology,Nantong,Jiangsu 226007

Keywords:

Euphorbia pulcherrima Wild; MS medium; phytohormone; differentiation

PACS:

S 68523036

DOI:

-

Abstract:

Using leaf and stem with axilla bud of Euphorbia pulcherrima Wild as the explant respectively, effects on the callus induction,the shoot differentiation and propagation,and the root differentiation of different phytohormone combition in MS basic medium were studied.The results showed that the best medium for callus induction was MS+BA 02 mg/L+NAA 05 mg/L,and the differentiation rate reached 925 %;The medium MS+BA 20 mg/L+NAA 02 mg/Lwas the most suitable for shoot induction than others,and the differentiation rate reached 833%;The medium with MS+BA 10 mg/L+NAA 02 mg/Lwas the best one for shoot propagation,the propagation rate was 108;Whereas,the best medium for root differentiation was 1/2 MS+BA 02 mg/L+NAA 05 mg/L,and the average root number per shoot was 35.This result would was beneficial to culture the Euphorbia pulcherrima Wild seedlings industrially.

References:

[1]徐建华,王代容,许志敏,等2010年11月各地盆花市场行情[J].中国花卉园艺,2011(1):41-42.

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