|Table of Contents|

Study on the Establishment of in vitro Regeneration System in Momordica charontia L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年02期
Page:
124-126
Research Field:
Publishing date:

Info

Title:
Study on the Establishment of in vitro Regeneration System in Momordica charontia L.
Author(s):
WU Peng1FANG Feng-xue2HUANG Ru-kui3HUANG Xiong-juan3CHEN Xiao-feng3
1.Guangxi Demonstration Park of Modern Agricultural Science and Technology,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007;
2.Sugarcane Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007;
3.Vegetable Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007
Keywords:
Momordica charantia.Lin vitro culturecallusadventitious bud
PACS:
S 6425036
DOI:
-
Abstract:
In vitro regeneration system of Momordica charantia L.based on MS medium with various concentration of hormone combinations were studied.The results showed that the most effective disinfection was reached when the clean peeled seeds were socked for 5 min,70% ethanol for 1 min and rinsing before HgCl2treatment for 10 min.Adventitious buds induction with callus was failed,while cotyledonary node reached 100% induction rate among all the parts of the sterile seedling and the optimum induction medium were MS+6-BA 2 mg/L+IBA 002 mg/L and MS+6-BA 4 mg/L+IBA 001 mg/L and the optimum root medium was 1/2MS+IBA 02 mg/L.

References:

[1]王国莉,李希陶.苦瓜离体再生体系建立的研究[J].安徽农业科学,2008,36(11):4408-4410.

[2]石若夫,王翔,王玮.长白大苦瓜高效再生系统的建立[J].生物技术,2007,17(4):89-91.
[3]冯锐,张慧英,刘百龙.苦瓜试管苗的快速繁殖[J].北方园艺,2007(3):165-166.
[4]潘绍坤,杜晓荣,屈小江.不同激素组合对苦瓜不定芽诱导的影响[J].安徽农业科学,2007,35(22):6760-6761.[5]李靖,李焕秀,李敏翠.翠妃苦瓜子叶不定芽诱导研究[J].北方园艺,2007(10):181-183.
[6]林义章,罗燕华,张志忠.苦瓜子叶节丛生芽的诱导[J].热带作物学报,2006,27(2):60-63.
[7]王小荣,刘选明,刘斌.不同激素组合对苦瓜离体快速繁殖的调控[J].湖南师范大学学报(自然科学版),2003,26(4):76-78.
[8]潘绍坤,王永清.苦瓜离体快速繁殖技术体系的研究[J].北方园艺,2006(4):148-150.
[9]宋莉英,谭诤,高峰.苦瓜愈伤组织诱导的多因子正交试验研究[J].西南师范大学学报,2004,29(3):462-465.[10]宣朴,岳春芳,李跃建,等.苦瓜愈伤组织再生植株[J].植物生理学通讯,2003,39(6):633.
[11]杨满业,赵茂俊,苗琛,等.苦瓜营养器官愈伤组织发生的比较研究[J].四川大学学报,2002,39(6):1155-1156.
[12]宣朴,陈新,岳春芳,等.苦瓜愈伤组织再生植株研究[J].西南农业学报,2006,19(5):940-942.
[13]唐琳,苟小平,陈放,等.用离体培养无性繁殖苦瓜[J].四川大学学报,1999,36(1):144-147.
[14]黄勇,汤青林,宋明.苦瓜组织培养体系的研究[J].西南农业学报,2007,20(4):860-863.
[15]王国莉,范红英,林润银,等.不同基因型苦瓜离体植株再生体系的建立[J].安徽农业科学,2008,36(28):12125-12127.
[16]宋莉英,高峰.苦瓜离体培养过程中内源激素含量的变化[J].植物学通迅,2006,23(2):192-196.
[17]张丽,李玉峰,代娟.苦瓜愈伤组织的诱导及培养条件优化[J].食品与生物技术学报,2007,26(3):116-120.

Memo

Memo:
-
Last Update: 2014-08-24