|Table of Contents|

Study on Polyploid Induction Technique of Walnut(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年02期
Page:
113-116
Research Field:
Publishing date:

Info

Title:
Study on Polyploid Induction Technique of Walnut
Author(s):
LIU Xi-xing1; GU Yu-hong2; LI Bao-guo1; QI Guo-hui1; ZHANG Xue-mei1; SHI Li-li1; SUN Meng1; NIU Bao-qing1; ZHANG Zhan-fang2
1.College of Forestry,Agricultural University of Hebei,Baoding,Hebei 071000;
2.College of Life Science,Agricultural University of Hebei,Baoding,Hebei 071000
Keywords:
walnut; colchicine; polyploid induction; chromosome identification
PACS:
S 6641036
DOI:
-
Abstract:
The‘Lvling’ walnut seeds,the annual ‘Lvling’ walnut seedling shoots and 5 years old ‘Lvling’ walnut scion nursery shoots were used as materials to induct polyploid plants by different colchicine solution concentrations and different treated time,the materials were identified by flow cytometry.The results showed that the variation rates were 0% respectively with the methods of shoot apex infiltration,shoot top-pinching infiltration,seeds kernel immersion,embryo immersion,kryptoblast stimulation and timulating axillary bud stimulation;with the method of seedlings top-pinchinginfiltration，there were 4 varied plants in 30 seedlings after infiltrated with 10% colchicine solution for 8 d,and the variation rate was 133%.The plants whose leaves were notably larger,thicker,darker and wrinkled than that of normal were identified,the result showed that 1 plant was tetraploid,3 plants were mixoploidy.

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Last Update: 2014-08-24