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Establishment of High Frequency Regeneration System of Alfalfa(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年02期
Page:
109-112
Research Field:
Publishing date:

Info

Title:
Establishment of High Frequency Regeneration System of Alfalfa
Author(s):
WANG Jing1; MA Dong-mei2; XU Xing2
1.College of Life Sciences,Ningxia University,Yinchuan,Ningxia 750021;
2.College of Agriculture,Ningxia University,Yinchuan,Ningxia 750021
Keywords:
alfalfa; explant; callus; high frequency regeneration system
PACS:
S 551+7
DOI:
-
Abstract:
The experiment gets across using ten kinds of the hypocotyl and cotyledon of alfalfa as explants were used to study the effect of different mediums,hormones in different concentrations on callus induction rate and differentiation rate.The results indicated that the Medicago sativa L.cv.Guochan hypocotyl callus differentiation rate was the highest;the best medium for callus induction was the MS salt+UM vitamin,2.0 mg/L 2,4-D+025 mg/L KT,2.0 g/L casein acid hydrolysate+6 g/L Agar+30 g/L sucrose;the best differentiation medium was the MS medium+05 mg/L KT+01 mg/LNAA;the best root medium for plant development was 1/2 MS.

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Last Update: 2014-08-24