Research on Callus Induction and Cell Suspension Culture of Rehmannia glutinosa f. hueichingensis (Chan et Sehih) Hsiao(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Research on Callus Induction and Cell Suspension Culture of Rehmannia glutinosa f. hueichingensis (Chan et Sehih) Hsiao

Author(s):: ZHANG Xiao-li¹; 2.; WANG Jian-jun¹; XU Shou-zhen¹; LI Jing-jing¹; CHEN Ming-xia¹; 2.; LI Ming-jun¹; 2.; 1.College of Life Science, Henan Normal University，Xinxiang，Henan 453007;?
2.Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs, University of Henan Province, Xinxiang, Henan 453007

Keywords:: Rehmannia glutinosa f. hueichingensis (Chan et Sehih) Hsiao; callus; cell suspension culture

Abstract:: The effects of inoculation method and plant growth regulator on callus induction and establishment of cell suspension culture system were studied. The rusults showed that when upper surface of leaf contact the medium, callus grows well on MS+2,4-D 1 mg/L+6-BA 0.4 mg/L, induction rate was 100%; on the same medium, callus could be divided into 3 types, callus of typeⅠwas straw yellow, bright particles, well dispersed and granular, fragile, this type of callus was suitable for cell suspension culture; on MS+2,4-D 3 mg/L＋6-BA 0.5 mg/L, the growth curve of suspension culture cells were ‘S’ type, cells dry weight was 4.94 g/L when cells cultured for 15 days.

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Last Update: 2014-08-23