Study on Rapid Propagation in vitro of Hosta plantaginea of Three New Species(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on Rapid Propagation in vitro of Hosta plantaginea of Three New Species

Author(s):: YANG Dan¹; GU De-feng¹; DONG Ran¹; ZHAO He-xiang¹; SUN Wei-yi²; 1.College of Horticulture,Jilin Agricultural University,Changchun,Jilin 130118;?
2.Kuancheng District Landscape Management Office of Changchun, Changchun, Jilin 130118

Abstract:: The young buds of H.‘Gloden Tiaia’, H.‘Gloden Gadet’and H.‘Gloden Eagle’were used as the explants to develop a protocol for adventitious buds induced. The successful establishment of five species of rapid propagation system. The results showed that the best medium for adventitious buds induced was MS+6-BA 2.5 mg/L+ NAA 0.2 mg/L; In the proliferation culture, the best proliferation medium for H.‘Gloden Tiaia’was MS+6-BA 2.0 mg/L+NAA 0.5 mg/L; The best proliferation medium for H.‘Gloden Gadet’was MS+6-BA 1.0 mg/L+NAA 0.2 mg/L; The best proliferation medium for H.‘Gloden Eagle’was MS+6-BA 4.0 mg/L+NAA 0.2 mg/L. Medium MS as H.‘Gloden Eagle’rooting medium, the rooting rate was over 100%; H.‘Gloden Gadet’in the rooting medium MS + NAA 0.5 mg/L;rooting rate was over 100%;H.‘Gloden Tiaia’in the rooting medium MS + NAA 0.3 mg/L; rooting rate was over 100%. The transplant survival rate of 100%.

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Last Update: 2014-08-23