REN Zhongjie,WANG Yu,PEI Haiying,et al.Study on Fitness of Beauveria bassiana Strain Bb170428 With 12 Low-toxic Insecticides[J].Northern Horticulture,2022,(18):29-36.[doi:10.11937/bfyy.20221114]
球孢白僵菌株Bb170428与十二种低毒杀虫剂混用适合度的研究
- Title:
- Study on Fitness of Beauveria bassiana Strain Bb170428 With 12 Low-toxic Insecticides
- Keywords:
- Beauveria bassiana; low-toxic pesticide; mixing; fitness
- 文献标志码:
- A
- 摘要:
- 以12种新型低毒杀虫剂为试材,以球孢白僵菌Bb170428为供试菌株,通过测量平板上孢子萌发、菌落生长直径和产孢量,评价新型低毒杀虫剂对球孢白僵菌Bb170428的影响,进而明确杀虫剂与白僵菌混用适合度,以期为白僵菌与杀虫剂合理混用提供参考依据。结果表明:虱螨脲和乙基多杀菌素对菌株Bb170428孢子萌发无明显影响,双丙环虫酯、茚虫威、氯虫苯甲酰胺、虱螨脲对孢子萌发、菌落生长和孢子产量影响较小,而螺虫?噻虫啉影响最大。菌药混合0 d茚虫威和双丙环虫酯推荐稀释倍数3倍和9倍,虱螨脲、氯虫苯甲酰胺、乙基多杀菌素、甲氨基阿维菌素苯甲酸盐和除虫脲推荐稀释倍数9倍对孢子萌发、菌落生长、产孢量无明显抑制作用;混合1 d双丙环虫酯推荐稀释倍数3倍和9倍、氯虫苯甲酰胺推荐稀释倍数9倍,混合3 d氯虫苯甲酰胺推荐稀释倍数9倍对菌株无明显影响。综上所述,杀虫剂浓度、菌药混合时长对菌株Bb170428孢子萌发、菌落生长速度和产孢量均能产生影响,大多杀虫剂随浓度增加和混合时间延长,对孢子萌发率、菌落生长和孢子产量的抑制作用增强,菌药混用适合度降低,但不同杀虫剂间存在较大差异。
- Abstract:
- Taking 12 new low-toxic insecticides as test materials and Beauveria bassiana Bb170428 as test strain,the effects of new low-toxic insecticides on Beauveria bassiana Bb170428 were evaluated by measuring spore germination,colony growth diameter and spore yield on the plate,so as to clarify the fitness of the mixture of insecticides and Beauveria bassiana,in order to provide reference for the rational mixture of Beauveria bassiana and insecticides.The results showed that lufenuron and spinetoram had no significant effect on spore germination of strain Bb170428,while afidopyropen,indoxacarb,chlorantranili-prole and lufenuron had little effect on spore germination,colony growth and spore yield,while spirotetramat?thiacloprid had the greatest effect.When Beauveria bassiana and insecticides were mixed for 0 day,indoxacarb and afidopyropen at the recommended dilution of 3 times and 9 times,and lufenuron,chlorantranili-prole,spinetoram,emamectin benzoate and hexaflumuron at the recommended dilution of 9 times had no obvious inhibitory effect on spore germination,colony growth and spore yield.Mixing Beauveria bassiana and insecticides for 1 day,afidopyropen at the recommended dilution of 3 times and 9 times,chlorantranili-prole at the recommended dilution of 9 times had no significant effect on the strains.Mixing Beauveria bassiana and insecticides for 3 days,chlorantranili-prole at the recommended dilution of 9 times had no significant effect on the strains.In summary,the concentration of pesticides and the mixing time of Beauveria bassiana and pesticides could affect the spore germination,colony growth rate and spore yield of strain Bb170428.With the increase of concentration and mixing time,most insecticides increased the inhibition of spore germination rate,colony growth and spore yield,and the fitness of Beauveria bassiana and insecticides decreased.However,there were great differences among different insecticides.
参考文献/References:
[1]PORTER J R.Agostino bassi bicentennial (1773-1973)[J].Bacteriological Reviews,1973,37(3):284-288.[2]张小霞,尹新明,梁振普.害虫生物防治技术基础与应用[M].北京:科学出版社,2010.[3]REHNER S A,MINNIS A M,SUNG G H,et al.Phylogeny and systematics of the anamorphic entomopathogenic genus Beauveria[J].Mycologia,2011,103(5):1055-1073.[4]王定锋,李良德,李慧玲,等.一株对茶丽纹象甲高毒力白僵菌菌株的筛选、鉴定与培养研究[J].茶叶科学,2021,41(1):101-112.[5]FARIA M R,WRAIGHT S P.Mycoinsecticides and Mycoacaricides:A comprehensive list with worldwide coverage and international classification of formulation types[J].Biology Control,2007,43(3):237-256.[6]宋龙腾,张鑫鑫,于洪春,等.卵孢白僵菌NEAU30503固态培养条件优化[J].植物保护,2016,42(2):123-128.[7]刘聪鹤,王雨薇,于洪春,等.卵孢白僵菌及添加杀虫助剂对两种地老虎的生物活性测定[J].应用昆虫学报,2016,53(4):739-744.[8]庄宝龙,赵薇,裴海英,等.白僵菌菌株分离培养及对四种重要害虫的致病力[J].应用昆虫学报,2020,57(6):1417-1426.[9]DUAN Y L,WU H,MA Z Y,et al..Scanning electron microscopy and histopathological observations of,Beauveria bassiana,infection of Colorado potato beetle larvae[J].Microbial Pathogenesis,2017,111(10):435-439.[10]徐文静,隋丽,高鹏,等.球孢白僵菌可湿性粉剂防治玉米螟的研究与应用[J].中国生物防治学报,2020,36(6):862-865.[11]巨修练.化学农药与生物农药的内涵与外延[J].农药,2011,50(2):153-154.[12]王峰,郑鹏飞,农向群,等.球孢白僵菌与三种农药对萝卜蚜的协同防治效果[J].中国生物防治学报,2017,33(6):752-759.[13]姜灵,洪波,王新谱,等.常用杀虫剂与球孢白僵菌的相容性及对温室白粉虱的协同防效[J].植物保护,2018,44(1):199-204.[14]于洪春,杜娟,宋龙腾,等.十种杀虫剂对布氏白僵菌孢子萌发和菌落生长的影响[J].中国生物防治学报,2016,32(4):449-455.[15]蔡悦,张胜利,李增智.球孢白僵菌与几种化学杀虫剂和除草剂的相容性[J].中国生物防治学报,2011,27(3):316-332.[16]王定锋,陈焰冠,李慧玲,等.叶蝉球孢白僵菌Bb305与8种化学农药的相容性研究[J].茶叶学报,2017,58(2):46-50.[17]许寿涛,应盛华,冯明光.十种常用农药与球孢白僵菌的生物学相容性[J].植物保护学报,2002,29(2):158-162.[18]曹伟平,宋健,冯书亮,等.球孢白僵菌与低剂量化学杀虫剂对小菜蛾的协同增效作用[J].中国生物防治学报,2018,34(3):370-376.[19]徐小俊,黄媛媛,晁骏,等.热带优势作物农药使用减量增效对策建议[J].中国植保导刊,2019,39(8):77-82.[20]周蒙.中国生物农药发展的现实挑战与对策分析[J].中国生物防治学报,2021,37(1):184-192.[21]郑永权,孙海滨,董丰收,等.高效低风险是农药发展的必由之路[J].植物保护,2012,38(2):1-3.[22]范晓杰,王琰,李晓莉,等.球孢白僵菌与5种农药的相容性及其对韭菜迟眼蕈蚊的联合毒力[J].中国生物防治学报,2017,33(4):496-503.
相似文献/References:
[1]宋龙腾,于洪春,王雨薇,等.卵孢白僵菌与农药混用对蛴螬防治效果研究[J].北方园艺,2013,37(01):131.
SONG Long-teng,YU Hong-chun,WANG Yu-wei,et al.Study on Control of Bettle Larvae by Mixture of Pesticides with Beauveria brongniartii[J].Northern Horticulture,2013,37(18):131.
[2]李 春 香,崔 也 平,张 英 英.卵孢白僵菌与农药的复配剂对甜菜夜蛾的毒力测定[J].北方园艺,2009,33(07):0.[doi:10.11937/bfyy.200907023]
LI Chun - xiang,CUI Ye - ping,ZHANG Ying - ying.Effect of the Mixture of Common Pesticides with Beauveria tenella Against Spodopteraexigua[J].Northern Horticulture,2009,33(18):0.[doi:10.11937/bfyy.200907023]
[3]何超,沈登荣,尹立红,等.三种低毒杀虫剂对井上蛀果斑螟的室内生物活性[J].北方园艺,2017,41(03):127.[doi:10.11937/bfyy.201703029]
HE Chao,SHEN Dengrong,YIN Lihong,et al.Indoor Bioactivities of Three Low-toxicity Insecticides Against Assara inouei Yamanaka[J].Northern Horticulture,2017,41(18):127.[doi:10.11937/bfyy.201703029]
备注/Memo
第一作者简介:任中杰(1997-),男,硕士研究生,研究方向为农业昆虫与害虫防治。E-mail:495120494@qq.com.责任作者:于洪春(1966-),男,博士,教授,现主要从事农业昆虫与害虫防治等研究工作。E-mail:hongcyu@126.com.基金项目:黑龙江省应用技术研究与开发计划重大资助项目(GA19B104)。收稿日期:2022-03-24