WANG Tingyue,LI Ning,HUANG Guohong,et al.Sterility Observation and Heterosis Analysis of Pepper CMS Line L9A[J].Northern Horticulture,2022,(10):9-16.[doi:10.11937/bfyy.20214001]
辣椒CMS不育系L9A的不育特性观察及杂种优势分析
- Title:
- Sterility Observation and Heterosis Analysis of Pepper CMS Line L9A
- Keywords:
- pepper; male sterility; heterosis; agronomic traits
- 文献标志码:
- A
- 摘要:
- 以课题组选育的CMS不育系L9A与其保持系L9B为试材，并以L9A为母本，22份优良自交系为父本配制杂交组合，研究分析了不育系和保持系形态性状差异、F1代11个农艺性状和5个品质性状的杂种优势，以期为辣椒育种实践提供参考依据。结果表明:L9A与L9B在株高、株幅、花药长度和花柱长度等方面存在明显差异；在22个辣椒杂交组合中，杂种优势普遍存在。L59、L90、L117等3个父本自交系可以使L9A的育性得到恢复，且其F1代在单株果数、单株产量和单果种子数的正向优势较为明显，同时发现F1代仅在1~2个品质性状上表现出正向杂种优势。相关性和杂种优势指数分析发现，L9A的F1代在果肉厚、株高、株幅、单果质量、可溶性糖含量、可溶性蛋白质含量等方面优势较大；单株产量与单株果数呈极显著正相关，各品质性状之间无显著相关关系。综上，在辣椒育种实践中，通过杂交育种提高辣椒的单株果数或单果质量，从而提高辣椒的单株产量，而利用杂种优势改良辣椒果实品质则存在一定困难。
- Abstract:
- CMS male sterile line L9A and its maintainer line L9B selected by the research group were used as test materials，and L9A was used as female parent and 22 excellent inbred lines were used as male parent to prepare hybrid combinations.The differences of morphological characters between male sterile lines and maintainer lines，and the heterosis of 11 agronomic characters and 5 quality characters in F1 generation were studied and analyzed,in order to provide reference for pepper breeding practice.The results showed that there were significant differences between L9A and L9B in plant height,plant width，stamen length and style length，and the heterosis was commonly observed in 22 pepper F1combinations.Three paternal inbred lines，L59，L90，and L117，could restore the fertility of L9A，and their F1 combinations displayed obviously positive heterosis in fruit number per plant，yield per plant，and seed number per fruit.At the same time，it was found that the positive heterosis of F1 generation was observed just in one or two quality traits.By the analysis of heterosis index,it was showed that the F1 generations of L9A had greater heterosis in pepper fruit flesh thickness，plant height，plant width，single fruit weight，soluble sugar content，and soluble protein content.By the correlationship analysis,a very significant positive correlation was presented between yield per plant and fruit number per plant，while no significant correlationship was observed between any two quality traits.In conclusion，in the practice of pepper breeding，the pepper yield in a single plant can be improved by heterosis breeding to increase the fruit number of single plant andsingle fruit weight，while it is difficult in some degree to improve the quality traits of pepper fruit through heterosis breeding.
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备注/Memo
第一作者简介：王艇越（1996-），男，硕士研究生，研究方向为蔬菜遗传育种。E-mail：18735417315@163.com.责任作者：逯明辉（1977-），男，博士，副教授，现主要从事蔬菜遗传育种与生物技术等研究工作。E-mail：xnjacklu@nwsuaf.edu.cn.基金项目：〖JP3〗陕西省重点研发计划资助项目（2019ZDLNY03-05）；〖JP〗财政部和农业农村部：国家大宗蔬菜产业技术体系西安综合试验站资助项目（CARS-23-G-22）。收稿日期：2021-09-27