[1]张永福,李利红,莫丽玲,等.茶多酚和咖啡因对缓解葡萄铝毒的生理机制[J].北方园艺,2018,42(08):1-6.[doi:10.11937/bfyy.20174536]
　ZHANG Yongfu,LI Lihong,MO Liling,et al.Physiological Mechanism of Tea Polyphenols and Caffeine on Relieving Aluminum Toxicity in Grape[J].Northern Horticulture,2018,42(08):1-6.[doi:10.11937/bfyy.20174536]
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茶多酚和咖啡因对缓解葡萄铝毒的生理机制

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年08 页码: 1-6 栏目: 出版日期: 2018-04-30
Title:
Physiological Mechanism of Tea Polyphenols and Caffeine on Relieving Aluminum Toxicity in Grape
作者:
张永福1; 李利红1; 莫丽玲1; 牛燕芬1; 王定斌2
1.昆明学院 农学院，云南 昆明 650214；2.宣威市阿都乡农业综合服务中心，云南 宣威 655425
Author(s):
ZHANG Yongfu1;  LI Lihong1;  MO Liling1;  NIU Yanfen1;  WANG Dingbin2
1.School of Agriculture, Kunming University, Kunming,Yunnan 650214； 2. Comprehensive Agricultural Service Center of Adu Township of Xuanwei City, Xuanwei, Yunnan 655425
关键词:
茶多酚; 咖啡因; 葡萄; 铝毒; 生理机制
Keywords:
tea polyphenol;  caffeine;  grape;  aluminum toxicity;  physiological mechanism
DOI:
10.11937/bfyy.20174536
文献标志码:
A
摘要:
以葡萄苗为试材，采用无土栽培的方法，研究了10 mmol·L-1硫酸铝钾胁迫下，0.25、0.50、0.75、1.00 mg·L-1的茶多酚和咖啡因处理对缓解葡萄铝毒的生理效应，以期为葡萄的耐铝栽培提供参考依据。结果表明：铝胁迫下葡萄生物量、叶绿素含量、根系活力下降，根冠比上升；叶片蛋白质、脯氨酸和过氧化氢的含量，及POD、SOD的活性上升；叶片和根系的丙二醛含量和氧自由基产生速率也上升。但在加入茶多酚和咖啡因后，葡萄生物量、叶绿素含量、根系活力、叶片POD和SOD活性，以及叶片和根系蛋白质和脯氨酸的含量均高于铝胁迫处理；而根冠比、叶片和根系的氧自由基产生速率、过氧化氢和丙二醛的含量则低于铝胁迫处理。茶多酚和咖啡因处理能够降低铝胁迫对葡萄植株生长的抑制作用，缓解铝毒对葡萄的伤害，效果最显著的是0.75 mg·L-1茶多酚和0.50 mg·L-1咖啡因处理。
Abstract:
The grape seedlings were used as materials, physiological effects of tea polyphenols and caffeine treatment of 0. 25 mg?L-1, 0. 50 mg?L-1, 0. 75 mg?L-1 and 1. 00mg?L-1, on relieving grape aluminum toxicity were studied by soilless cultivation under 10 mmol?L-1 aluminum sulfate stress, in order to provide reference basis for aluminum tolerance cultivation of grape. The results showed that under the aluminum stress, the biomass, chlorophyll content, root activity and root shoot ratio of grape increased; the contents of protein, proline and hydrogen peroxide in leaves, and the activities of POD and SOD increased; the leaves and roots of malondialdehyde content and oxygen free radical production rate also rose. But after adding tea polyphenols and caffeine, grape biomass, chlorophyll content, root activity, POD and SOD activity in leaves, and the content of protein and proline in leaves and roots were higher than that of aluminum stress; and the root shoot ratio, the oxygen free radical generation rate, the contents of hydrogen peroxide and malondialdehyde in leaves and roots were lower than that of aluminum stress. Tea polyphenols and caffeine treatment can reduce the inhibitory effect on the growth of grape plant aluminum stress, alleviate Al toxicity on grape damage. Among them, the most significant effect is 0.75 mg?L-1 polyphenols and 0.50 mg?L-1 caffeine treatment.

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备注/Memo

第一作者简介：张永福（1981-），男，云南弥勒人，博士，教授，现主要从事果树抗性生理等研究工作。E-mail: 123017360@qq.com.基金项目：国家自然科学基金资助项目（31660559）；云南省应用基础研究计划资助项目（2017FD087）；昆明学院科学研究资助项目（XJZZ1604）。收稿日期：2018-02-05

更新日期/Last Update: 2018-02-28