MAO Xiujie,WANG Shuai,WANG Yu,et al.Genetic Difference of Plant Type Characteristics and SSR Makers Associated With Pruning Inflorescence Number of Two Self-pruning Cultivar Tomatoes[J].Northern Horticulture,2018,42(21):1-6.[doi:10.11937/bfyy.20174510]
番茄封顶花序节位的遗传差异及SSR分子标记研究
- Title:
- Genetic Difference of Plant Type Characteristics and SSR Makers Associated With Pruning Inflorescence Number of Two Self-pruning Cultivar Tomatoes
- Keywords:
- tomato; self-pruning; genetic difference; self-pruning inflorescence number; SSR (simple sequence repeat)
- 文献标志码:
- A
- 摘要:
- 以封顶花序节位不同的番茄品系‘GXF’和‘AXF’为试材，对植株花序数、果实品质等性状进行分析，设计封顶花序节位SSR引物检测遗传多样性，以明确2份试验材料间存在的遗传差异并初步获得与目标性状连锁的分子标记。常规方法测定2个番茄品系的花序数、果实品质、叶片类型等性状，并设计255对SSR引物对基因组进行多态性分析。结果表明：番茄品系‘GXF’的封顶花序数、株高均极显著高于番茄品系‘AXF’，2个品系在叶片类型、叶片形状、节间距、果实熟性、果实颜色、果实形状、果形指数、果实硬度、可溶性固形物含量及单果质量等指标均差异不显著；从255对SSR引物中共筛选出4对具有多态性的引物。2个自封顶番茄品系间封顶花序数、株高的遗传差异极显著，其它各性状遗传差异较小，检测出4个与封顶花序数相关联的SSR分子标记。
- Abstract:
- In order to make clear the genetic differences and obtain molecular markers linked to target trait, two self-pruning cultivar tomatoes ‘GXF’ and ‘AXF’ with different inflorescence nodes were used as experimental materials to study the difference of inflorescence number, fruit characteristics and plant traits and detect the genetic diversity by designing SSR primers. Influence number, fruit characteristics and plant traits were detected by conventional method and 255 pairs of SSR primers were designed for polymorphism analysis. The results showed that the number of pruning inflorescence and plant height of ‘GXF’ were significantly higher than that of ‘AXF’, and there wsas no significant difference in leaf type, leaf shape, node spacing, fruit maturity, fruit color, fruit shape, fruit shape index, fruit hardness, soluble solids, and single-fruit weight between the two inbred lines, as well as 4 pairs of SSR primers were screened with polymorphism. There were significantly differences in pruning inflorescence number and plant height between the two self-pruning cultivar tomatoes and had little difference in other characteristics, and 4 SSR markers were obtained that associated with pruning inflorescences number.
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备注/Memo
第一作者简介:毛秀杰(1971-)，女，博士，教授，研究方向为蔬菜遗传育种及分子生物学。E-mail:maoxiujie@126.com.基金项目：河北省自然基金资助项目（C2015407085）；河北省园艺作物育种应用技术研发中心资助项目；河北省现代农业产业技术体系蔬菜创新团队建设资助项目（QN2015101）。收稿日期：2018-05-08