[1]任梦楠,金 玉,李晓雁,等.荒漠化土壤中菌株ZMN-3的分离鉴定及其保水效果[J].北方园艺,2018,42(08):3.[doi:10.11937/bfyy.20174354]
　REN Mengnan,JIN Yu,LI Xiaoyan,et al.Isolation, Identification and Water-retaining Effect of ZMN-3 From Desertified Soil[J].Northern Horticulture,2018,42(08):3.[doi:10.11937/bfyy.20174354]
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荒漠化土壤中菌株ZMN-3的分离鉴定及其保水效果

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年08 页码: 3 栏目: 出版日期: 2018-04-30
Title:
Isolation, Identification and Water-retaining Effect of ZMN-3 From Desertified Soil
作者:
任梦楠1 ; 金 玉1 ; 李晓雁2 ; 李思琪1 ; 王艳梅1 ; 黄海东1
1. 天津农学院 农学与资源环境学院，天津 300384；2. 天津农学院 食品科学与生物工程学院，天津 300384
Author(s):
REN Mengnan 1; JIN Yu 1; LI Xiaoyan 2; LI Siqi 1; WANG Yanmei 1; Huang Haidong 1
1.College of Agronomy and Resources and Environment, Tianjin Agricultural University, Tianjin 300384; 2.Department of Food Science and Biotechnology, Tianjin Agricultural University, Tianjin 300384
关键词:
胞外多糖; 马西利亚菌属; 鉴定; 保水效果
Keywords:
extracellular polysaccharide;  Massilia;  identification;  water retention effect
DOI:
10.11937/bfyy.20174354
文献标志码:
A
摘要:
以荒漠化土壤为试材，采用稀释平板和多相分类鉴定法，分离和鉴定了胞外多糖合成菌ZMN-3，并研究了该菌株的发酵液性能和保水效果，以期为荒漠化土壤的人工生物结皮固沙提供菌种资源和参考依据。结果表明：菌株ZMN-3属于马西利亚菌属（Massilia），其16S rRNA序列与Massilia plicata 76T的同源性最高（96.5%），结合系统进化和生理生化分析，判断ZMN-3应为马西利亚菌属的一个新种。菌株ZMN-3合成的胞外多糖包括2个组分，重均分子量Mw分别为1.91×104 kDa和947 kDa；30 ℃发酵3 d，ZMN-3发酵液粘度达到2 150 mPa?s，呈粘稠的假塑性流体，喷洒于沙土表面后形成致密的生物结皮，具有明显的保水效果。b
Abstract:
Desertified soil was used as experimental material. Polysaccharides synthetic strain ZMN-3 was isolated and identified by the method of dilution flat and polyphasic taxonomy. For the purpose of supply bacteria resources and reference for applying in artificial biological crust fix sand, properties of fermentation broth and water conservation of strain ZMN-3 were investigated. The results showed that strain ZMN-3 belonged to the genes of Massilia, exhibiting the highest 16S rRNA sequence similarity with Massilia plicata 76T(96.5%). On the basis of phylogenetics, physiological and biochemical analysis, strain ZMN-3 was considered to represent a novel species of the genus Massilia. The extracellular polysaccharide synthesized by ZMN-3 consisted of two components with different molecular weight distribution. The weight average molecular weight Mw was 1.91×104 kDa and 947 kDa, respectively. After incubation at 30 ℃ for 3 days, the viscosity of ZMN-3 fermentation broth reached 2 150 mPa?s, as viscous pseudoplastic fluid. The microbial agent of ZMN-3 was sprayed on the surface of sand, compact bio-crust formed and the ability of water conservation of sand increased.

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备注/Memo

第一作者简介：任梦楠（1992-），女，硕士研究生，研究方向为微生物学。E-mail：1375331479@qq.com.责任作者：黄海东（1972-），男，博士，教授，研究方向为微生物资源的开发与利用。E-mail：hhaidong@126.com.基金项目：国家自然科学基金面上资助项目（31571790）；天津农学院研究生培养质量提升资助项目（2017YJG009）。收稿日期：2018-02-02

更新日期/Last Update: 2018-03-01