QIAO Yu,HU Liping,ZHENG Yanyan,et al.Effects of Low Temperature Storage on Postharvest Physiological and Regulation Mechanism of Glucose Metabolism of Okra[J].Northern Horticulture,2025,(7):49-57.[doi:10.11937/bfyy.20243679]
低温贮藏对秋葵采后生理及糖代谢调控的影响
- Title:
- Effects of Low Temperature Storage on Postharvest Physiological and Regulation Mechanism of Glucose Metabolism of Okra
- 文章编号:
- 1001-0009(2025)07-0049-09
- Keywords:
- okra; low temperature; quality; glucose metabolism
- 分类号:
- S 649
- 文献标志码:
- A
- 摘要:
- 以秋葵为试材,采用低温贮藏对比试验的方法,研究了不同低温(10、4 ℃)对秋葵贮藏期间营养品质和糖代谢的影响,以期为秋葵的贮藏保鲜提供参考依据。结果表明:在10 ℃贮藏条件下,贮藏后期秋葵的叶绿素、β-胡萝卜素和玉米黄质素含量有所增加;能够有效减少淀粉、可溶性蛋白质、可溶性糖以及相对电导率的增加;可减少蔗糖、果糖和葡萄糖含量的降低;并且对蔗糖合成酶-分解方向(sucrose synthase,SS-I)活性起到抑制作用。而在4 ℃贮藏后期,秋葵出现了冷害症状,其细胞膜通透性增大,蔗糖含量持续下降,叶绿素、β-胡萝卜素和玉米黄质素含量也急剧下降。在整个贮藏期内,4 ℃贮藏下秋葵的淀粉和可溶性糖含量相较22 ℃贮藏时更高,但麦芽糖含量始终保持在最低水平。综上,10 ℃低温贮藏更有助于维持秋葵采后的品质。
- Abstract:
- Taking okra as the test material,the effects of different low temperatures (4,10 ℃,and 22 ℃) on the nutritional quality and sugar metabolism of okra during storage were studied by means of a low-temperature storage comparison test,in order to provide reference for the storage and preservation of okra.The results showed that under the storage condition of 10 ℃,the contents of chlorophyll,β-carotene and zeaxanthin in okra increased in the later stage of storage;it could effectively reduce the increase of starch,soluble protein,soluble sugar and relative conductivity;it could reduce the decrease of sucrose,fructose and glucose contents;and it could inhibit the activity of sucrose synthase-decomposition direction (sucrose synthase;SS-I).However,in the later stage of storage at 4 ℃,cold damage symptoms appeared in okra,with increased cell membrane permeability,continuous decrease of sucrose content,and sharp decline of chlorophyll,β-carotene and zeaxanthin contents.Throughout the storage period,the contents of starch and soluble sugar in okra stored at 4 ℃ were higher than those stored at 22 ℃,but the content of maltose remained at the lowest level.In conclusion,10 ℃ low-temperature storage is more conducive to maintaining the postharvest quality of okra.
参考文献/References:
[1]SERENO A B,DAYANE P C,ANTUNES A F,et al.Effects of okra (Abelmoschus esculentus (L.) Moench) on glycemic markers in animal models of diabetes:A systematic review[J].Journal of Ethnopharmacology,2022,298:115544.[2]TEMAM N,MOHAMMED W,AKLILU S.Variabilityassessment of okra (Abelmoschus esculentus (L.) Moench) genotypes based on their qualitative traits[J].International Journal of Agronomy,2021,2021(1):6678561.[3]WU D T,NIE X R,SHEN D D,et al.Phenolic compounds,antioxidant activities,and inhibitory effects on digestive enzymes of different cultivars of okra (Abelmoschus esculentus)[J].Molecules,2020,25(6):1276.[4]ROMDHANE M H,CHAHDOURA H,BARROS L,et al.Chemical composition,nutritional value,and biological evaluation of Tunisian okra pods (Abelmoschus esculentusL.Moench)[J].Molecules,2020,25(20):4739.[5]APOLINRIO D S A P,NETO J X D S,MOURA L F W G,et al.Okra (Abelmoschus esculentus L.Moench) fruit powder standardized in flavonoids improves glycemic control and metabolic memory in acute and chronic hyperglycemia[J].Food Bioscience,2024,61:104870.[6]FATIMA M,RAKHA A,ALTEMIMI A B,et al.Okra:Mucilage extraction,composition,applications,and potential health benefits[J].European Polymer Journal,2024,215:113193.[7]QIAO Y,ZHENG Y Y,WATKINS C B,et al.Transcriptomic and metabolomic analysis of quality deterioration of postharvest okra fruit at different storage temperatures[J].Postharvest Biology and Technology,2024,218:113146.[8]马丽丽,白春美,周新原,等.低温贮藏对高品质番茄果实采后生理的影响[J].北方园艺,2023(14):97-104.[9]陈廷慧,曾加庆,罗炜华,等.不同贮藏温度对黄秋葵采后品质及木质化的影响[J].保鲜与加工,2023,23(10):7-14.[10]毕莹,王新宇,李慧,等.不同品种哈密瓜果实脯氨酸代谢对低温的响应[J].食品科学,2024,45(6):216-224.[11]王香兰,薛洁,李欢,等.低温和膜袋包装对瑞玉猕猴桃果实贮藏品质的影响[J].西北农林科技大学学报(自然科学版),2021,49(11):79-90.[12]乔沛,殷菲胧,李静,等.百香果采后贮藏保鲜研究进展[J].保鲜与加工,2020,20(4):220-225,229.[13]LIU Y F,LIU Y F,CHEN Q M,et al.Methyljasmonate treatment alleviates chilling injury and improves antioxidant system of okra pod during cold storage[J].Food Science & Nutrition,2023,11(4):2049-2060.[14]辛松林,秦文,李慧妍,等.不同贮藏温度和保鲜处理对黄秋葵贮藏品质及生理的影响[J].保鲜与加工,2019,19(5):59-65.[15]ZHANG L H,XU Y,LUO Z X,et al.Overexpression of a transcription factor MdWRKY126 altered soluble sugar accumulation in apple and tomato fruit[J/OL].Horticultural Plant Journal,(2024-07-01)[2024-06-19].https://doi.org/10.1016/j.hpj.2023.09.010.[16]CAO K F,WEI Y Y,CHEN Y,et al.PpCBF6 is a low-temperature-sensitive transcription factor that binds the PpVIN2 promoter in peach fruit and regulates sucrose metabolism and chilling injury[J].Postharvest Biology and Technology,2021,181:111681.[17]LYU N,ZHANG H Y,ZHOU H T,et al.Hot water mobilizes the metabolism of energy,solublesugar,cellwall,and phenolics to cope with chilling injury in postharvest snap beans[J].Journal of the Science of Food and Agriculture,2024,104(13):8263-8274.[18]陈琪琪,杨洋,郭丽红,等.果蔬低温贮藏的糖代谢转录调控研究进展[J].食品研究与开发,2023,44(8):207-212.[19]YU J Q,LIU X L,WANG W Y,et al.MdCIbHLH1 modulates sugar metabolism and accumulation in apple fruits by coordinating carbohydrate synthesis and allocation[J].Horticultural Plant Journal,2025,11(2):578-592.[20]NING M,TANG F,ZHANG Q,et al.The quality of Gold Queen Hami melons stored under different temperatures[J].Scientia Horticulturae,2019,243:140-147.[21]XU F,WANG H F,TANG Y C,et al.Effect of 1-methylcyclopropene on senescence and sugar metabolism in harvested broccoli florets[J].Postharvest Biology and Technology,2016,116:45-49.[22]刘峥颢,庞艳苹,杨丽萍.巨鹿杏中β-胡萝卜素的HPLC分析及多地域杏果中含量比对[J].现代食品,2021,27(14):217-222.[23]曹建康,姜微波,赵玉梅.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社,2007.[24]陈楚英,刘善军,简火仔,等.不同采收期对‘新余蜜桔’采后生理特性和耐贮性的影响[J].中国农学通报,2019,35(27):58-64.[25]CAI N,WAN C P,CHEN J Y,et al.Evaluation of postharvest storability of Ponkan mandarins stored at different temperatures[J].Folia Horticulturae,2021,33(2):354-364.[26]XIAO X,YANG M J,DONG W Q,et al.Gibberellic acid inhibited chlorophyll degradation in post-harvest okras[J].Postharvest Biology and Technology,2022,190:111951.[27]KULTYS E,KUREK M A.Green extraction of carotenoids from fruit and vegetable byproducts:A review[J].Molecules,2022,27(2):518.[28]柳婷婷,应金耀,尉吉乾,等.不同品种(系)黄秋葵果实营养成分的比较分析[J].分子植物育种,2024(2):1-9.[29]JIANG M J,YANG Y Y,WEI W,et al.Interaction of MaERF11 with an E3 ubiquitin ligase MaRFA1 is involved in regulation of banana starch degradation during postharvest ripening[J].Horticultural Plant Journal,2025,11(2):608-618.[30]KANWAL R,ASHRAF H,SULTAN M,et al.Effect of 1-methyl cyclopropane and modified atmosphere packaging on the storage of okra (Abelmoschus esculentus L.):Theory and experiments[J].Sustainability,2020,12(18):7547.[31]FINGER F L,DELLA-JUSTINA M E,CASALI V W D,et al.Temperature and modified atmosphere affect the quality of okra[J].Scientia Agricola,2008,65(4):360-364.[32]张碧青,林恒进,徐怀德.不同贮藏温度对黄秋葵保鲜效果的影响研究[J].中国果菜,2019,39(11):1-8,19.[33]王露凡.采后桃果实冷害形成与糖代谢的关联性研究[D].杭州:浙江工商大学,2023.[34]王海丹,普红梅,杨芳,等.不同贮藏温度下油麦菜品质变化及其货架期预测[J].食品研究与开发,2022,43(15):38-47.[35]康慧芳,乔勇进,王晓,等.不同保鲜剂处理对“巨峰”葡萄货架期品质的影响[J].保鲜与加工,2020,20(6):6-13.[36]汪竹苇.麝香草酚微胶囊复合保鲜膜液对秋葵贮藏品质的影响[D].雅安:四川农业大学,2023.[37]LIU B D,JIAO W X,WANG B G,et al.Near freezing point storage compared with conventional low temperature storage on apricot fruit flavor quality (volatile,sugar,organic acid) promotion during storage and related shelf life[J].Scientia Horticulturae,2019,249:100-109.[38]ZHU Y,HUANG Q,PAN Y G,et al.Abnormal behavior of chilling injury in postharvest Papaya fruit is associated with sugar metabolism[J].Journal of Food Science,2022,87(3):919-928.[39]WANG J W,DONG S Z,JIANG Y G,et al.Influence of long-term cold storage on phenylpropanoid and soluble sugar metabolisms accompanied with peel browning of ‘Nanguo’ pears during subsequent shelf life[J].Scientia Horticulturae,2020,260:108888.[40]李姚瑶.p-香豆酸处理调控采后桃果实冷害的机理研究[D].西安:西北大学,2020.
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备注/Memo
第一作者简介:乔宇(1998-),女,硕士研究生,研究方向为农产品加工与贮藏。E-mail:17320676209@163.com.责任作者:王正荣(1978-),女,博士,副教授,现主要从事农产品加工与贮藏等研究工作。E-mail:wazhro@qq.com.基金项目:北京市农林科学院创新能力建设专项基金资助项目(KJCX20230212,KJCX20230309,KJCX20230418,KJCX20240508)。收稿日期:2024-07-24