YANG Shuo,HOU Qiumei,ZHOU Yan,et al.Research on Metabonomics of New Floribunda Rose Cultivar‘Huahao Yueyuan’ at Different Flowering Stages[J].Northern Horticulture,2025,(3):80-87.[doi:10.11937/bfyy.20243255]
月季新品种‘花好月圆’不同花期的代谢研究
- Title:
- Research on Metabonomics of New Floribunda Rose Cultivar‘Huahao Yueyuan’ at Different Flowering Stages
- 文章编号:
- 1001-0009(2025)03-0080-08
- Keywords:
- cultivar ‘Huahao Yueyuan’; different flowering periods; untargeted metabonomics; metabolites differential
- 分类号:
- S 685.12
- 文献标志码:
- A
- 摘要:
- 以‘花好月圆’月季初开期和盛开期的花朵为试材，采用液相色谱-质谱联用(LC-MS)技术对‘花好月圆’不同花期花朵进行非靶向代谢组学分析，研究了不同花期对月季花朵代谢物的差异影响，以期为科学开发利用该种月季提供参考依据。结果表明：月季新品种‘花好月圆’不同花期的花朵中共检测筛选出485个代谢物。采用|log2(FoldChange)|≥1、P≤0.05、VIP＞1，得出正离子模式下差异代谢物为18种（上调6种，下降12种），主要显著富集在氨酰基tRNA生物合成、苯丙氨酸代谢、精氨酸等合成途径上。负离子模式下差异代谢物为11种（上调6种，下降5种），显著富集在戊糖和葡萄糖醛酸相互转化、抗坏血酸和阿糖二酸代谢、磷酸戊糖等合成途径上。综上所述，月季新品种‘花好月圆’花朵不同时期的代谢物种类差异明显，初开期的代谢物主要为羧酸及其衍生物、吲哚及其衍生物、孕烯醇酮脂类等。盛开期的代谢物主要为有机氧化合物。与初花期对比，盛花期的苯丙氨酸、脯氨酸、异亮氨酸、腺苷及多种糖类等化合物显著下调，说明盛开期较初开期花朵香味减弱、功能性成分减少、光合作用能力减弱。
- Abstract:
- Taking the initial blooming and full blooming flowers of ‘Huahao Yueyuan’ as the test materials，and used liquid chromatographyass spectrometry (LC-MS) technology to perform untargeted metabolomics analysis on flowers of different blooming periods of this variety,the effects of different blooming periods on the metabolites of rose flowers were studied，in order to provide reference for the scientific development utilization of this rose species.The results showed that a total of 485 metabolites were detected and screened from flowers of different flowering periods of the new rose variety ‘Huahao Yueyuan’.Using | log2 (FoldChange) | ≥ 1，P≤ 0.05，VIP>1，18 differential metabolites were identified in the positive ion mode (6 up-regulated and 12 down regulated)，mainly significantly enriched in the biosynthesis pathways of aminoacyl tRNA，phenylalanine metabolism，arginine，etc.Under negative ion mode，there were 11 differentially expressed metabolites (6 up-regulated and 5 down regulated)，significantly enriched in the conversion of pentose and glucuronic acid，ascorbic acid and arabic acid metabolism，and pentose phosphate synthesis pathways.In summary，the new variety of roses‘Huahao Yueyuan’ had significant differences in the types of metabolites at different stages of flowering.The metabolites at the initial opening stage were mainly carboxylic acids and their derivatives，indoles and their derivatives，pregnenolone lipids，etc.The metabolites during the blooming period were mainly organic oxygen compounds.Compared with the initial flowering period，compounds such as phenylalanine，proline，isoleucine，adenosine，and various sugars were significantly downregulated during the peak flowering period，indicating a decrease in flower aroma，functional components，and photosynthetic capacity during the blooming period.
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备注/Memo
第一作者简介：杨朔（1995-），女，硕士，助理研究员，现主要从事植物保育与应用等研究工作。E-mail:3082327547@qq.com.责任作者：吴洪娥（1983-），女，硕士，高级工程师，现主要从事蔷薇属植物种质资源收集及产业化应用等研究工作。E-mail:271448209@qq.com.基金项目：贵州省高层次创新人才（百层次）资助项目（黔科合平台人才［2018］5635）；贵州特色经济植物育种创新能力建设资助项目（黔科合服企［2024］004-1）。收稿日期：2024-08-13