[1]方茹,罗优波,胡亚芬,等.基于SRAP分子标记的五十份木槿种质资源亲缘关系的分析[J].北方园艺,2025,(1):8-14.[doi:10.11937/bfyy.20242577]
　FANG Ru,LUO Youbo,HU Yafen,et al.Analysis the Phylogenetic Relationship of 50 Germplasm Resources of Hibiscus syriacus L.Based on SRAP Molecular Markers[J].Northern Horticulture,2025,(1):8-14.[doi:10.11937/bfyy.20242577]
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基于SRAP分子标记的五十份木槿种质资源亲缘关系的分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2025年1 页码: 8-14 栏目: 出版日期: 2025-01-15
Title:
Analysis the Phylogenetic Relationship of 50 Germplasm Resources of Hibiscus syriacus L.Based on SRAP Molecular Markers
文章编号:
1001-0009(2025)01-0008-07
作者:
方茹1; 罗优波2; 胡亚芬2; 王衍彬1; 王丽玲1; 杨少宗1
（1.浙江省林业科学研究院，森林食品资源利用与质量控制国家林业局重点实验室，浙江 杭州 310023；2.杭州市林业和湿地科学研究院，浙江 杭州 310022）
Author(s):
FANG Ru1; LUO Youbo2; HU Yafen2; WANG Yanbin1; WANG Liling1; YANG Shaozong1
(1.Zhejiang Academy of Forestry/Key Laboratory of State Forest Food Resources Utilization and Quality Control，Hangzhou，Zhejiang 310023；2.Hangzhou Forest and Wetland Science Research Institute，Hangzhou，Zhejiang 310022)
关键词:
木槿; SRAP分子标记; 种质资源; 亲缘关系
Keywords:
Hibiscus syriacus L.; SRAP molecular markers; germplasm resources; phylogenetic relationship
分类号:
S 688.9
DOI:
10.11937/bfyy.20242577
文献标志码:
A
摘要:
以木槿（Hibiscus syriacus L.）为试材，采用 UPGMA法,通过16对SRAP引物组合研究50份木槿种质亲缘关系，以期为加速木槿新品种的选育进程提供参考依据。结果表明：16对SRAP引物组合共扩增出390条条带，其中有388条具有多态性，多态性比率平均为99.49%。在遗传距离为0.362 0 处，50 份木槿样本被分为 5组，分类结果显示木槿品种间的亲缘关系与木槿花花瓣基部色斑和花瓣型这2个形态特征呈现密切相关，而与花色以及植株、叶片以及果实等形态特征未呈现相关性。综上所述，SRAP标记技术对木槿基因组检测多态性水平高，可用于木槿种质资源鉴定以及分子标记辅助育种等工作。
Abstract:
Taking Hibiscus syriacus L.as the test material,UPGMA method was adopted,the genetic relationships of 50 Hibiscus syriacus L.were analyzed by 16 pairs of SRAP primers,in order to provide reference for accelerating the breeding process of new Hibiscus syriacus L.varieties.The results showed that 16 SRAP combining primers produced 390 bands，included 388 polymorphic bands，with a polymorphism ratio of 99.49%.At the genetic distance of 0.362 0，50 Hibiscus syriacus L. varieties were classified into 5 categories.The results of clustering analysis revealed significant correlation among phylogenetic relationship of Hibiscus syriacus L. varieties and two morphological characteristics of flower，basal blotches in the petal and petal-type.And yet，there was no significant correlation between phylogenetic relationship and flower color.In addition，there was no significant correlation between phylogenetic relationship and the morphological characteristics of plant，leaf and fruit.In conclusion，the SRAP markers，showing high level of polymorphism in the genome detection of Hibiscus syriacus L.，could be used as an effective means for identification of the germplasm resources and molecular marker-assisted breeding of Hibiscus syriacus L.

参考文献/References:

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备注/Memo

第一作者简介：方茹（1977-），女，博士，副研究员，现主要从事森林食品等研究工作。E-mail:fangru7756@163.com.责任作者：杨少宗（1974-），男，博士，副研究员，现主要从事林木遗传育种等研究工作。E-mail:yangsz863@163.com.基金项目：浙江省省属科研院所专项资助项目 （2024F1065-4）；浙江省省级林业专项资助项目（20211231Y085）。收稿日期：2024-06-20

更新日期/Last Update: 2025-01-16