TANG Jixiang,ZHANG Junfeng,LIU Mengyu,et al.Analysis of Salt-alkali Tolerance of Wild Type and Hair Root Gshdz4 Transformation in Trifolium pratense[J].Northern Horticulture,2025,(5):74-82.[doi:10.11937/bfyy.20242368]
野生型和发根转化Gshdz4红车轴草耐盐碱性分析
- Title:
- Analysis of Salt-alkali Tolerance of Wild Type and Hair Root Gshdz4 Transformation in Trifolium pratense
- 文章编号:
- 1001-0009(2025)05-0074-09
- Keywords:
- Leguminosae; Trifolium pratense; salt and alkali resistance; Gshdz4
- 分类号:
- S 541+.2
- 文献标志码:
- A
- 摘要:
- 以红车轴草为试材，利用40、80、120、160、320 mmol·L-1NaCl溶液和20、40、60、80、160 mmol·L-1NaHCO3溶液分别对红车轴草种子和幼苗进行处理，研究了盐碱胁迫下红车轴草种子的发芽势、根长等生长指标，观察胁迫下0、4、8、20、24、32、44、48 h和52 h植物表型，测定受害植株比例，以期为近缘改良植物耐碱性和培育耐碱嵌合体红车轴草提供参考依据。结果表明：野生型能耐受80 mmol·L-1盐胁迫和40 mmol·L-1碱胁迫。将耐碱基因Gshdz4-GFP通过K599发根农杆菌转入红车轴草植株根系，利用激光共聚焦检测绿色荧光蛋白信号，发现Gshdz4基因表达于红车轴草根的外层细胞。该研究测定了未转基因和转基因根系超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量，结果显示转Gshdz4提高植株根系对碱胁迫耐性。
- Abstract:
- Taking seeds of Trifolium pratense as the test material，the methods of treating the seeds and seedlings of Trifolium pratense with 40，80，120，160，and 320 mmol·L-1 as well as NaHCO3 solutions at concentrations of 20，40，60，80,and 160 mmol·L-1 were used.Various growth parameters included germination potential and root length were examined under these stress conditions.Plant phenotypes were observed at different time points (0，4,8,24，32，44，48 hours and 52 hours),and the proportion of affected plants was determined,in order to provide insights into improving alkaline tolerance and breeding alkali-resistant chimera Trifolium pratense varieties.The results showed that the wild type could tolerate salt stress up to a concentration of 80 mmol·L-1and alkali stress up to a concentration of 40 mmol·L-1.The alkali-tolerant gene GSHDZ4-GFP was introduced into the root system of Trifolium pratense using Agrobacterium K599.The expression pattern of the Gshdz4 gene in Trifolium pratense roots was detected using laser confocal microscopy by observing green fluorescent protein signals.Super oxide dismutase (SOD) activity and malondialdehyde (MDA) contents were measured in both non-transgenic and transgenic roots.The results indicated that transplantation of Gshdz4 increased the tolerance of plant roots to alkali stress.
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备注/Memo
第一作者简介：唐继祥（2001-），男，硕士研究生，研究方向为豆科花卉耐盐碱机制。E-mail:jxtang1019@163.com.责任作者：曹蕾（1990-），女，博士，讲师，硕士生导师，现主要从事豆科花卉耐盐碱机制和植物开花机制等研究工作。E-mail:leicao@neau.edu.cn.基金项目：黑龙江省自然科学基金资助项目（LH2021C058）；哈尔滨学院青年博士科研启动基金资助项目(HUDF2019104)；黑龙江省大学生创新训练资助项目（202210234027）；国家自然青年科学基金资助项目（32001505）。收稿日期：2024-06-04