WANG Pengbo,YANG Maolin,FENG Guojun,et al.Effects of Abscisic Acid on the Germination of Phaseolus vulgaris Seeds Under Low Temperature Stress[J].Northern Horticulture,2024,(24):15-21.[doi:10.11937/bfyy.20241997]
低温胁迫下脱落酸对菜豆种子萌发的影响
- Title:
- Effects of Abscisic Acid on the Germination of Phaseolus vulgaris Seeds Under Low Temperature Stress
- 文章编号:
- 1001-0009(2024)24-0015-07
- Keywords:
- low temperature stress; abscisic acid; seed germination
- 分类号:
- S 643.1
- 文献标志码:
- A
- 摘要:
- 以菜豆品种“热那亚”为试材，采用水培萌发的方法，设定CK（常温对照）、LT(低温处理)和低温胁迫下9个不同浓度的ABA处理，分别为100、75、50、25、10、5、1、0.5 mg·L-1和0.1 mg·L-1，通过统计种子发芽率、测定生理指标，研究了不同浓度ABA处理对低温胁迫下菜豆种子萌发的影响和外源脱落酸对低温胁迫下菜豆种子萌发的缓解作用,以期为菜豆的耐低温研究提供参考依据。结果表明：在0.1 mg·L-1ABA浸种〖JP2〗处理能提高低温胁迫下菜豆种子的发芽率、发芽势、胚轴长度，提高抗氧化酶活性，增强种子清除体内活性氧（ROS）和丙二醛（MDA）的能力；提高低温胁迫下菜豆种子内的脯氨酸和可溶性蛋白质含量。
- Abstract:
- Taking the Phaseolus vulgaris variety ‘Genoa’ as the experimental material，hydroponic germination method was employed.Eleven treatments were set up，including nine different concentrations of ABA (100，75，50，25，10，5，1，0.5 mg·L-1 and 0.1 mg·L-1)，CK (normal temperature control)，LT (low temperature treatment)，the effects of different concentrations of ABA on seed germination and the alleviating effects of exogenous abscisic acid on seed germination under low temperature stress were studied.Statistical analysis of seed germination rate and determination of physiological indexes were conducted，in order to provide reference for studying the low temperature tolerance of beans.The results showed that soaking bean seeds in a solution containing 0.1 mg·L-1 ABA improved their germination rate，potential，cotyledon length as well as antioxidant enzyme activity.Additionally，it enhanced their ability to scavenge reactive oxygen species (ROS) and malondialdehyde (MDA) under low temperature stress conditions.Furthermore，proline and soluble protein contents in bean seeds increased when exposed to low temperature stress.
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备注/Memo
第一作者简介：王鹏博（2001-），男，硕士研究生，研究方向为菜豆逆境调控生理及分子机制。E-mail:wong_2001pb@126.com.责任作者：刘畅（1989-），女，博士，副研究员，现主要从事园艺植物遗传育种与生物技术等研究工作。E-mail:lcky0909@126.com.基金项目：黑龙江省重点研发计划资助项目（SC2022ZX02C0202）；农业农村部政府购买合同资助项目（19240656，152408033）；2022年度省属高校基本科研业务费资助项目（2022-KYYWF-1071)。收稿日期：2024-05-13