[1]张月盈,李枝林,和凤美,等.观赏用微型兰花组织培养最佳配方的优化[J].北方园艺,2024,(13):49-57.[doi:10.11937/bfyy.20234380]
　ZHANG Yueying,LI Zhilin,HE Fengmei,et al.Optimization of the Best Formula for Tissue Culture of Ornamental Miniature Orchid[J].Northern Horticulture,2024,(13):49-57.[doi:10.11937/bfyy.20234380]

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观赏用微型兰花组织培养最佳配方的优化

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2024年13 页码: 49-57 栏目: 出版日期: 2024-07-15

Title:: Optimization of the Best Formula for Tissue Culture of Ornamental Miniature Orchid

文章编号:: 1001-0009(2024)13-0049-09

作者:: 张月盈1; 李枝林1; 和凤美1; 李叶芳1; 王玉英1; 瞿素萍2; （1.云南农业大学园林园艺学院，云南昆明 650201；2.云南省农业科学院花卉研究所，云南昆明 650205）

Author(s):: ZHANG Yueying1; LI Zhilin1; HE Fengmei1; LI Yefang1; WANG Yuying1; QU Suping2; (1.College of Gardening and Horticulture,Yunnan Agricultural University,Kunming,Yunnan 650201;2.Flower Research Institute of Yunnan Academy of Agricultural Sciences,Kunming,Yunnan 650205)

关键词:: 兰花; 微型培养; 培养基; 生长; 观赏应用

Keywords:: orchid; micro-culture; medium; growth; viewing applications

分类号:: S 682.31

DOI:: 10.11937/bfyy.20234380

文献标志码:: A

摘要:: 以大花蕙兰‘大凤’×豆瓣兰‘太极圣梅’（Cymbidium hybridium ‘Dafeng’×C.serratum var.goeringii ‘Taijishengmei’）F1代试管苗为试材，采用在培养基中添加不同的有机物质，筛选最适生长的培养基；并在此基础上，在培养基中添加不同色素，研究其对植株生长的影响及观赏效果，以期为兰花微型培养的观赏应用研究提供参考依据。结果表明：在1/2 MS+IBA 0.5 mg·L-1+6-BA 2 mg·L-1+碳粉0.5 g·L-1的培养基中，添加75 mL·L-1椰汁有利于兰花试管苗叶长、叶宽、株高以及鲜质量的增加，对植株生长效果最好；在1/2 MS+IBA 0.5 mg·L-1+6-BA 2 mg·L-1+碳粉0.5 g·L-1+椰汁75 mL·L-1的培养基中添加不同浓度的食用色素红曲红、叶绿素铜钠盐、栀子蓝，经121 ℃高温灭菌后培养基的颜色均会发生变化。叶绿素铜钠盐浓度为533.3 mg·L-1的彩色培养基植株生长良好，培养基整体观赏效果最佳。

Abstract:: The F1 test-tube seedlings of Cymbidium hybridium ‘Dafeng’×C.serratum var.goeringii ‘Taijishengmei’ were used as the test materials.Different organic substances were added to the medium to screen the optimal growth medium.On this basis,different pigments were added to the medium to study their effects on plant growth and ornamental effects,in order to provide reference for the study of ornamental application of orchid microculture.The results showed that in the medium of 1/2MS+IBA 0.5 mg·L-1+6-BA 2 mg·L-1+carbon powder 0.5 g·L-1,added 75 mL·L-1 coconut juice was benefited to the increased of leaf length,leaf width,plant height and fresh weight of orchid plantlets in vitro,and had the best effect on plant growth.In 1/2MS+IBA 0.5 mg·L-1+6-BA 2 mg·L-1+carbon powder 0.5 g·L-1+coconut juice 75 mL·L-1 medium added different concentrations of edible pigment monascus red,sodium copper chlorophyllin,gardenia blue,after 121 ℃ high temperature sterilization medium color was changed.The plants grew well in the color medium with the concentration of sodium copper chlorophyllin of 533.3 mg·L-1,and the overall ornamental effect of the medium was the best.

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备注/Memo

第一作者简介：张月盈（1999-），女，硕士研究生，研究方向为兰花栽培。E-mail：2513459540@qq.com.责任作者：王玉英（1980-），女，博士，副教授，现主要从事观赏植物资源利用与创新等研究工作。E-mail：wyysxp@126.com.基金项目：云南省重大科技专项资助项目（202102AE090052）；云南省基础研究资助项目（202001AT070101）；云南省创新团队资助项目（202105AE160012）。收稿日期：2023-12-14

更新日期/Last Update: 2024-07-18